Invitrogen

Bovine Serum Albumin Polyclonal Antibody

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Bovine Serum Albumin Antibody in Western Blot (WB) — Western blot analysis was performed by loading 500 ng (Lane 1), 250 ng (Lane 2), 125 ng (Lane 3) and 60 ng (Lane 4) of BSA. The blot was probed with Anti- Bovine Serum Albumin Rabbit Polyclonal Antibody (Product # A11133, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 69 kDa band corresponding to Bovine Serum Albumin was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Bovine Serum Albumin Antibody — Immune complex of DQ™ BSA conjugate (Product # D12050, D12051) with an anti-bovine serum albumin (BSA) antibody (Product # A11133) for the fluorescent detection of the Fc receptor-mediated phagocytosis pathway. The DQ™ BSA is a derivative of BSA that is labeled to such a high degree with either the green-fluorescent BODIPY® FL or red-fluorescent BODIPY® TR-X dye that the fluorescence is self-quenched. Upon binding to an Fc receptor, the nonfluorescent immune complex is internalized and the protein is subsequently hydrolyzed to fluorescent peptides within the phagovacuole.

Bovine Serum Albumin Antibody in Immunocytochemistry (ICC/IF) — Published figure using Bovine Serum Albumin polyclonal antibody (Product # A11133) in Immunocytochemistry

Product Details

A11133

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 9 publications 9 publications
Immunohistochemistry (Paraffin) (IHC (P))	Assay-dependent	-
Immunocytochemistry (ICC/IF)	Assay-dependent	View 3 publications 3 publications
ELISA (ELISA)	Assay-dependent	-
Immunoprecipitation (IP)	Assay-dependent	View 1 publication 1 publication
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Bovine
Published species	Baboon, Bovine, Human, Mouse, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Bovine Serum Albumin
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	IgG fraction
Storage buffer	PBS, pH 7.2
Contains	5mM sodium azide
Storage conditions	4° C
RRID	AB_2534139

Product Specific Information

Anti-bovine serum albumin (anti-BSA) antibody is a highly purified rabbit IgG fraction that can be used for the detection of BSA in tracer studies or in protease assays. This antibody demonstrates high specificity for BSA and little or no crossreactivity to other serum proteins. Cat. No. A11133 can be used in enzyme and fluorescence immunoassays and blots, cell or tissue immunostaining, and as a precipitating antibody or as an immuno-adsorbant when attached to solid supports. For detection of this rabbit antibody, we offer a variety of second-step reagents labeled with biotin or with a wide range of fluorophores.

Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. We suggest using serial dilutions from about 1:100 to 1:1,000 to determine the optimal conditions for your experiments.

Target Information

Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Nanostructure of bioactive glass affects bone cell attachment via protein restructuring upon adsorption.

Scientific reports

Thamma U,Kowal TJ,Falk MM,Jain H

A11133 was used in Western Blot to provide new insight into the interaction of cells with the scaffolds' interfacial layer, which is vital for the continued development of engineered tissue scaffolds.

Thu Mar 11 00:00:00 EST 2021

Serum proteins are extracted along with monolayer cells in plasticware and interfere with protein analysis.

Journal of biological methods

Hong X,Meng Y,Kalkanis SN

A11133 was used in western blot to investigate the enrichment of serum proteins when extracting proteins from plastic ware

Thu Oct 01 00:00:00 EDT 2020

SUMOylating Two Distinct Sites on the A-type Potassium Channel, Kv4.2, Increases Surface Expression and Decreases Current Amplitude.

Frontiers in molecular neuroscience

Welch MA,Forster LA,Atlas SI,Baro DJ

A11133 was used in Western Blotting to show that Kv4.2 channels are SUMOylated and that SUMOylation can independently regulate Kv4.2 surface expression and IA Gmax in opposing directions.

Tue Sep 29 00:00:00 EDT 2020

Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer.

International journal of molecular sciences

Krstić Ristivojević M,Grundström J,Apostolović D,Radomirović M,Jovanović V,Radoi V,Kiewiet MBG,Vukojević V,Ćirković Veličković T,van Hage M

A11133 was used in Western Blotting to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer.

Tue Aug 11 00:00:00 EDT 2020

Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer.

International journal of molecular sciences

Krstić Ristivojević M,Grundström J,Apostolović D,Radomirović M,Jovanović V,Radoi V,Kiewiet MBG,Vukojević V,Ćirković Veličković T,van Hage M

A11133 was used in Western Blotting to investigate how protein glycosylation by α-Gal affects the susceptibility to gastric digestion and transport through the Caco-2 cell monolayer.

Tue Aug 11 00:00:00 EDT 2020

African Swine Fever Virus Protein pE199L Mediates Virus Entry by Enabling Membrane Fusion and Core Penetration.

mBio

Matamoros T,Alejo A,Rodríguez JM,Hernáez B,Guerra M,Fraile-Ramos A,Andrés G

A11133 was used in Western Blot to characterize the African swine fever virus protein pE199L as a contributor to host cell entry.

Tue Aug 11 00:00:00 EDT 2020

African Swine Fever Virus Protein pE199L Mediates Virus Entry by Enabling Membrane Fusion and Core Penetration.

mBio

Matamoros T,Alejo A,Rodríguez JM,Hernáez B,Guerra M,Fraile-Ramos A,Andrés G

A11133 was used in Western Blot to characterize the African swine fever virus protein pE199L as a contributor to host cell entry.

Tue Aug 11 00:00:00 EDT 2020

Growth Differentiation Factor 11 treatment leads to neuronal and vascular improvements in the hippocampus of aged mice.

Scientific reports

Ozek C,Krolewski RC,Buchanan SM,Rubin LL

A11133 was used in Western Blotting to study the effects of Growth Differentiation Factor 11 on the hippocampus of aged mice.

Fri Nov 23 00:00:00 EST 2018

Towards single molecule biosensors using super-resolution fluorescence microscopy.

Biosensors & bioelectronics

Lu X,Nicovich PR,Gaus K,Gooding JJ

A11133 was used to perform a proof-of-principle experiment to demonstrate that antibody-antigen binding events can be monitored at the single molecule level using single molecule localization microscopy

Sat Jul 15 00:00:00 EDT 2017

Human dental pulp stem cells derived from cryopreserved dental pulp tissues of vital extracted teeth with disease demonstrate hepatic-like differentiation.

Journal of tissue engineering and regenerative medicine

Chen YK,Huang AH,Chan AW,Lin LM

Published figure using Bovine Serum Albumin polyclonal antibody (Product # A11133) in Immunofluorescence

Wed Jun 01 00:00:00 EDT 2016

Sphingosine 1-phosphate and its carrier apolipoprotein M in human sepsis and in Escherichia coli sepsis in baboons.

Journal of cellular and molecular medicine

Frej C,Linder A,Happonen KE,Taylor FB,Lupu F,Dahlbäck B

A11133 was used in Western Blotting to evaluate changes in sphingosine-1-phosphate and its carrier protein, apolipoprotein M, during sepsis.

Wed Jun 01 00:00:00 EDT 2016

Reversible S-nitrosylation limits over synthesis of fungal styrylpyrone upon nitric oxide burst.

Applied microbiology and biotechnology

Zhao Y,He M,Xi Q,Ding J,Hao B,Keller NP,Zheng W

A11133 was used in western blot to characterize the limits of over synthesis of fungal styrylpyrone upon nitric oxide burst due to reversible S-nitrosylation

Sun May 01 00:00:00 EDT 2016

A DNA-dependent protease involved in DNA-protein crosslink repair.

Cell

Stingele J,Schwarz MS,Bloemeke N,Wolf PG,Jentsch S

A11133 was used in western blot to show that Wss1 is crucial for cell survival upon exposure to formaldehyde and topoisomerase 1-dependent DNA damage

Thu Jul 17 00:00:00 EDT 2014

LTBP-2 competes with tropoelastin for binding to fibulin-5 and heparin, and is a negative modulator of elastinogenesis.

Matrix biology : journal of the International Society for Matrix Biology

Sideek MA,Menz C,Parsi MK,Gibson MA

A11133 was used in Binding experiments to investigate the modulating effect of latent transforming growth factor-beta-1 binding protein-2 on two key interactions of tropoelastin during elastinogenesis.

Sat Feb 01 00:00:00 EST 2014

CR1-mediated ATP release by human red blood cells promotes CR1 clustering and modulates the immune transfer process.

The Journal of biological chemistry

Melhorn MI,Brodsky AS,Estanislau J,Khoory JA,Illigens B,Hamachi I,Kurishita Y,Fraser AD,Nicholson-Weller A,Dolmatova E,Duffy HS,Ghiran IC

Published figure using Bovine Serum Albumin polyclonal antibody (Product # A11133) in Immunofluorescence

Fri Oct 25 00:00:00 EDT 2013

Double nanohole optical trapping: dynamics and protein-antibody co-trapping.

Lab on a chip

Zehtabi-Oskuie A,Jiang H,Cyr BR,Rennehan DW,Al-Balushi AA,Gordon R

A11133 was used in immunoprecipitation to study the dynamics of trapped particles.

Sun Jul 07 00:00:00 EDT 2013

Imaging the endothelial glycocalyx in vitro by rapid freezing/freeze substitution transmission electron microscopy.

Arteriosclerosis, thrombosis, and vascular biology

Ebong EE,Macaluso FP,Spray DC,Tarbell JM

A11133 was used in immunocytochemistry to utilize rapid freezing/freeze substitution transmission electron microscopy for imaging of the endothelial glycocalyx in vitro