Invitrogen

Brachyury Recombinant Rabbit Monoclonal Antibody (ARC1983)

View all (17) Brachyury antibodies

Questions & Answers

Questions & Answers

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Brachyury Antibody in Western Blot (WB) — Western blot analysis of extracts of NCI-H460 cells using Brachyury Recombinant Monoclonal Antibody (Product # MA5-38134) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Brachyury Antibody in Western Blot (WB) — Western blot analysis of Brachyury using Brachyury Recombinant Monoclonal Antibody (Product # MA5-38134) at a 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

MA5-38134

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	ARC1983
Immunogen	A synthesized peptide derived from human Brachyury
Conjugate	Unconjugated
Form	Liquid
Concentration	0.78 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Positive Samples: NCI-H460, Mouse brain, Mouse thymus, Rat brain

Target Information

The protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: brachyury homolog; Brachyury protein; brachyury-like 2; brachyury-like 3; low ratio; Protein T; RP11-459F1.1; T brachyury homolog; T, brachyury homolog; T-box transcription factor T

Gene Aliases: Bra; cou; D17Mit170; Low; Lr; me75; SAVA; T; T1; TBXT; TFT; Tl2; Tl3

UniProt ID: (Human) O15178, (Mouse) P20293

Entrez Gene ID: (Human) 6862, (Rat) 292301, (Mouse) 20997

Molecular Function: Rel homology transcription factor

negative regulation of transcription from RNA polymerase II promoter vasculogenesis somitogenesis neural plate morphogenesis neural tube closure heart morphogenesis positive regulation of transcription from RNA polymerase II promoter involved in myocardial precursor cell differentiation transcription, DNA-templated signal transduction penetration of zona pellucida mesoderm development mesoderm migration involved in gastrulation positive regulation of cell proliferation anterior/posterior axis specification, embryo notochord formation signal transduction involved in regulation of gene expression BMP signaling pathway post-anal tail morphogenesis embryonic skeletal system development canonical Wnt signaling pathway bone morphogenesis SMAD protein signal transduction determination of heart left/right asymmetry cellular response to retinoic acid primitive streak formation regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter anatomical structure morphogenesis anterior/posterior pattern specification reproductive process notochord development positive regulation of transcription from RNA polymerase II promoter regulation of transcription, DNA-templated multicellular organism development cardiac muscle cell differentiation

Process(es)

RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription factor binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function sequence-specific DNA binding DNA binding protein binding