|Tested species reactivity||Chemical|
|Published species reactivity||Hamster|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100 - 1:200|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
OMA1-06200 detects bromodeoxyuridine (BrdU).
OMA1-06200 has been successfully used in immunocytochemistry, immunohistochemical and FACS procedures. This antibody specifically detects free BrdU and BrdU that has been incorporated into nuclear DNA.
OMA1-06200 antigen is purified BrdU.
Store at 4°C, or in small aliquots at -20°C.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Myosin16b: The COOH-tail region directs localization to the nucleus and overexpression delays S-phase progression.
OMA1-06200 was used in immunohistochemistry to study the role of the myosin16b COOH-tail region in localization to the nucleus and S-phase progression
|Cameron RS,Liu C,Mixon AS,Pihkala JP,Rahn RJ,Cameron PL||Cell motility and the cytoskeleton (64:19)||2007|