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|Tested species reactivity||Chem|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Mouse / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Nuclear envelope protein Lem2 is required for mouse development and regulates MAP and AKT kinases.
B35130 was used in immunohistochemistry - frozen section to characterize mice harboring a disrupted allele for the Lem2 gene
|Tapia O,Fong LG,Huber MD,Young SG,Gerace L||PloS one (10:null)||2015|
RASSF1A inactivation unleashes a tumor suppressor/oncogene cascade with context-dependent consequences on cell cycle progression.
B35130 was used in immunocytochemistry to study the involvement of RASSF1A inactivation in carcinogenesis
|Ram RR,Mendiratta S,Bodemann BO,Torres MJ,Eskiocak U,White MA||Molecular and cellular biology (34:2350)||2014|
|Not Applicable||Not Cited||
Cell-cycle analyses using thymidine analogues in fission yeast.
B35130 was used in immunocytochemistry to evaluate the effectiveness of thymidine analogues for cell cycle studies in fission yeast
|Anda S,Boye E,Grallert B||PloS one (9:null)||2014|
The rostral migratory stream plays a key role in intranasal delivery of drugs into the CNS.
B35130 was used in immunohistochemistry to discuss the anatomical structures involved in the transport of intranasally administered drugs into the central nervous system.
|Scranton RA,Fletcher L,Sprague S,Jimenez DF,Digicaylioglu M||PloS one (6:null)||2011|