Jurkat T-cell leukemia cells were treated with 10µM BrdU for 1.5 hours. The cells were then fixed in ETOH and stored overnight at =-20°C. An acid denaturation method was used to prepare the cells before labeling with BrdU mouse monoclonal antibody (Clone MoBU-1) Alexa Fluor® 488 *for flow cytometry* *100 tests* to detect the incorporated BrdU, each using 5µl antibody conjugate labeling 1 x 10^6 cells. Proliferating cells are clearly distinguished from non-proliferating cells.
|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
The Fanconi Anemia Pathway Protects Genome Integrity from R-loops.
B35139 was used in flow cytometry to elucidate how genome integrity is protected from R-loops in the fanconi anemia pathway
|García-Rubio ML,Pérez-Calero C,Barroso SI,Tumini E,Herrera-Moyano E,Rosado IV,Aguilera A||PLoS genetics (11:null)||2015|
YAP regulates S-phase entry in endothelial cells.
B35139 was used in flow cytometry to study the role of YAP in cell proliferation and survival using different cell types
|Shen Z,Stanger BZ||PloS one (10:null)||2015|
|Not Applicable||Not Cited||
Basal p21 controls population heterogeneity in cycling and quiescent cell cycle states.
B35139 was used in flow cytometry to analyze how population heterogeneity in quiescent and cycling cell cycle states is controlled by basal p21
|Overton KW,Spencer SL,Noderer WL,Meyer T,Wang CL||Proceedings of the National Academy of Sciences of the United States of America (111:E4386)||2014|
Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair.
B35139 was used in flow cytometry to determine deubiquitinases required to assemble homologous recombination factors BRCA1 and RAD51
|Yu H,Pak H,Hammond-Martel I,Ghram M,Rodrigue A,Daou S,Barbour H,Corbeil L,Hébert J,Drobetsky E,Masson JY,Di Noia JM,Affar el B||Proceedings of the National Academy of Sciences of the United States of America (111:285)||2014|
Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1.
B35139 was used in flow cytometry to examine the diagnostic and prognostic properties of miR-96 expression levels in prostate cancer cohorts.
|Haflidadóttir BS,Larne O,Martin M,Persson M,Edsjö A,Bjartell A,Ceder Y||PloS one (8:null)||2013|
|Not Applicable||Not Cited||
Thermally targeted p21 peptide enhances bortezomib cytotoxicity in androgen-independent prostate cancer cell lines.
B35139 was used in flow cytometry to examine the combination treatment of prostate cancer cells with bortezomib and the C-terminal part of the p21(WAF1/CIP1) protein bound to the Elastin-like polypeptide carrier.
|Mikecin AM,Walker LR,Kuna M,Raucher D||Anti-cancer drugs (25:189)||2014|