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Jurkat T-cell leukemia cells were treated with 10µM BrdU for 1.5 hours. The cells were then fixed in ETOH and stored overnight at =-20°C. An acid denaturation method was used to prepare the cells before labeling with BrdU mouse monoclonal antibody (Clone MoBU-1) Alexa Fluor® 488 *for flow cytometry* *100 tests* to detect the incorporated BrdU, each using 5µl antibody conjugate labeling 1 x 10^6 cells. Proliferating cells are clearly distinguished from non-proliferating cells.
|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG|
|Conjugate||Alexa Fluor® 488|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 2 publications below|
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
YAP regulates S-phase entry in endothelial cells.
B35139 was used in flow cytometry to study the role of YAP in cell proliferation and survival using different cell types
|Shen Z,Stanger BZ||PloS one (10:null)||2015|
Upregulation of miR-96 enhances cellular proliferation of prostate cancer cells through FOXO1.
B35139 was used in flow cytometry to examine the diagnostic and prognostic properties of miR-96 expression levels in prostate cancer cohorts.
|Haflidadóttir BS,Larne O,Martin M,Persson M,Edsjö A,Bjartell A,Ceder Y||PloS one (8:null)||2013|