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|Tested species reactivity||Chem|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG|
|Conjugate||Alexa Fluor® 594|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Featured Article: Nuclear export of opioid growth factor receptor is CRM1 dependent.
B35132 was used in immunocytochemistry to identify the nuclear export signal for opioid growth factor receptor.
|Kren NP,Zagon IS,McLaughlin PJ||Experimental biology and medicine (Maywood, N.J.) (241:273)||2016|
Pax9 regulates a molecular network involving Bmp4, Fgf10, Shh signaling and the Osr2 transcription factor to control palate morphogenesis.
B35132 was used in immunocytochemistry to investigate the role of Osr2 and Pax9 in palate development.
|Zhou J,Gao Y,Lan Y,Jia S,Jiang R||Development (Cambridge, England) (140:4709)||2013|
VEGFR-3 controls tip to stalk conversion at vessel fusion sites by reinforcing Notch signalling.
B35132 was used in immunohistochemistry to show that macrophage-derived VEGF-C activates VEGFR-3 in tip cells to reinforce Notch signaling.
|Tammela T,Zarkada G,Nurmi H,Jakobsson L,Heinolainen K,Tvorogov D,Zheng W,Franco CA,Murtomäki A,Aranda E,Miura N,Ylä-Herttuala S,Fruttiger M,Mäkinen T,Eichmann A,Pollard JW,Gerhardt H,Alitalo K||Nature cell biology (13:1202)||2011|