|Tested species reactivity||Chemical|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG|
|Storage buffer||PBS, pH 7.2|
|Contains||5mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical, thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Cell surface glycoengineering improves selectin-mediated adhesion of mesenchymal stem cells (MSCs) and cardiosphere-derived cells (CDCs): Pilot validation in porcine ischemia-reperfusion model.
B35141 was used in flow cytometry to discuss and investigate methods of cellular glycoengineering.
|Lo CY,Weil BR,Palka BA,Momeni A,Canty JM,Neelamegham S||Biomaterials (74:19)||2016|
MERIT40 cooperates with BRCA2 to resolve DNA interstrand cross-links.
B35141 was used in flow cytometry to study resolution of DNA interstrand cross-links by cooperation of BRCA2 and MERIT40
|Jiang Q,Paramasivam M,Aressy B,Wu J,Bellani M,Tong W,Seidman MM,Greenberg RA||Genes and development (29:1955)||2015|
Tumor suppressor and deubiquitinase BAP1 promotes DNA double-strand break repair.
B35141 was used in flow cytometry to determine deubiquitinases required to assemble homologous recombination factors BRCA1 and RAD51
|Yu H,Pak H,Hammond-Martel I,Ghram M,Rodrigue A,Daou S,Barbour H,Corbeil L,Hébert J,Drobetsky E,Masson JY,Di Noia JM,Affar el B||Proceedings of the National Academy of Sciences of the United States of America (111:285)||2014|
Notch3 signaling gates cell cycle entry and limits neural stem cell amplification in the adult pallium.
B35141 was used in immunohistochemistry (free floating) to examine the role of Notch3 in neuronal stem cells.
|Alunni A,Krecsmarik M,Bosco A,Galant S,Pan L,Moens CB,Bally-Cuif L||Development (Cambridge, England) (140:3335)||2013|