Immunostaining of Brg1 by BRG-01 antibody. A - Brg1-negative SW13 cells were transfected with Brg1-expression plasmid and analysed by Western blotting for Brg1 expression. B - HEK293 cytoplasmic (cyt) and nuclear (nuc) lysates were analysed by Western blotting for Brg1 presence.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Bacterially produced GST-fused protein representing 242 C-terminal amino acids of human Brg1.|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Western Blot (WB)||10 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes an epitope within C-terminal part of human Brg1.
Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.