|ChIP assay (ChIP)||Assay-dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 248 and 443 of Human Brn2|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-30124 targets Brn2 in IHC (P), ChIP, ICC, IP and WB applications and shows reactivity with Human and mouse samples.
The PA5-30124 immunogen is recombinant fragment corresponding to a region within amino acids 248 and 443 of Human Brn2.
POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, that occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1; MIM 164175) and Oct2 (POU2F2; MIM 164176) and the pituitary protein Pit1 (PIT1; MIM 173110). Class III POU genes are expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression (Schreiber et al., 1993 [PubMed 8441633]; Atanasoski et al., 1995 [PubMed 7601453]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Brain-2; brain-2 class III POU-domain protein; Brain-specific homeobox/POU domain protein 2; brn-2; BRN2; class 3; Nervous system-specific octamer-binding transcription factor N-Oct-3; Oct-7; OCT7; Octamer-binding protein 7; Octamer-binding transcription factor 7; OTF-7; OTF7; POU domain; POU domain, class 3, transcription factor 2; POUF3; transcription factor 2
Gene Aliases: 9430075J19Rik; A230098E07Rik; Brn-2; BRN2; N-Oct3; oct-7; OCT7; OTF-7; OTF7; POU3F2; POUF3
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