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Bub1 Antibody (MA5-42550) in WB
Western blot analysis of Bub1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. Bub1 Monoclonal Antibody (Product # MA5-42550) at 1:500 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysate. Lane 2: K562 cell lysate. Lane 3: 293T cell lysate.
Product Details
MA5-42550
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
A6E10
Immunogen
Recombinant protein within human Bub1 aa 1-200
Conjugate
Form
Liquid
Concentration
2 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911691
Product Specific Information
Positive Control: Hela cell lysate, K562 cell lysate, 293T cell lysate.
Subcellular Location: Nucleus, kinetochore.
Target Information
BUB1A is a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BUB1 budding uninhibited by benzimidazoles 1 homolog; BUB1, mitotic checkpoint serine/threonine kinase; BUB1A; budding uninhibited by benzimidazoles 1 homolog; hBUB1; Mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase
Gene Aliases: BUB1; BUB1A; BUB1L; hBUB1
UniProt ID: (Human) O43683
Entrez Gene ID: (Human) 699
protein phosphorylation
apoptotic process
sister chromatid cohesion
regulation of sister chromatid cohesion
mitotic nuclear division
mitotic cell cycle checkpoint
mitotic spindle assembly checkpoint
cell proliferation
viral process
cell division
regulation of chromosome segregation
spindle assembly checkpoint
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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