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Invitrogen
C/EBP alpha Monoclonal Antibody (15C8)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-825
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
15C8
Immunogen
Synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-825 detects C/EBP alpha protein in human, mouse and rat samples.
MA1-825 has successfully been used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 45 kDa protein representing C/EBP alpha in transfected hu293T cells. MA1-825 is specific to the amino terminal region of C/EBP alpha.
The MA1-825 immunogen is a synthetic peptide corresponding to residues M(1) E S A D F Y E V E P R P P(14) of mouse C/EBP alpha. This peptide (Cat. # PEP-242) is available for use in neutralization and control experiments.
Target Information
The C/EBP (CCAAT/enhancer binding protein) family of transcription factors--alpha, beta, delta, and epsilon)--are widely expressed proteins found in a variety of tissues. These domains contain an evolutionarily conserved five-amino acid motif (the regulatory domain motif RDM) that conforms to the consensus sequence (I/V/L) KXEP. One of the major roles of these transcription factors is that of metabolic regulation in liver and fat cells. Studies suggest that these transcription factors act in a cascade aiding in the development of the adipose cell. Three C/EBP beta isoforms (LAP*, LAP, and LIP) arise from different translation initiation and display different function in gene regulation. From a neurological standpoint, C/EBPs have been implicated in the mediation of nerve growth factor, altering synaptic plasticity, and mediation of inflammatory response in AD patients.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C EBP; C/EBP alpha; CAAT/enhancer-binding protein, DNA-binding protein; CCAAT/enhancer binding protein (C/EBP), alpha; OTTHUMP00000220269; unnamed protein product
Gene Aliases: C/EBP-alpha; C/ebpalpha; CBF-A; CEBP; DBPCEP
UniProt ID: (Human) P49715, (Rat) P05554, (Mouse) P53566
Entrez Gene ID: (Human) 1050, (Rat) 24252, (Mouse) 12606
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
RNA polymerase I regulatory region sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
kinase binding
chromatin DNA binding
identical protein binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
HMG box domain binding
STAT family protein binding
DNA-binding transcription factor binding
transcription factor binding
protein domain specific binding
histone deacetylase binding
macromolecular complex binding
negative regulation of transcription from RNA polymerase II promoter
liver development
generation of precursor metabolites and energy
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase I promoter
negative regulation of cell proliferation
positive regulation of gene expression
cytokine-mediated signaling pathway
myeloid cell differentiation
lung development
granulocyte differentiation
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
glucose homeostasis
positive regulation of macrophage activation
fat cell differentiation
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of inflammatory response
regulation of cell cycle
lipid homeostasis
interleukin-6-mediated signaling pathway
white fat cell proliferation
energy homeostasis
integrated stress response signaling
positive regulation of DNA-templated transcription, initiation
urea cycle
embryonic placenta development
mitochondrion organization
Notch signaling pathway
cholesterol metabolic process
macrophage differentiation
regulation of cell proliferation
positive regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of transcription, DNA-templated
cell maturation
inner ear development
white fat cell differentiation
brown fat cell differentiation
cellular response to lithium ion
cellular response to tumor necrosis factor
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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