Invitrogen

C/EBP beta Polyclonal Antibody

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C/EBP beta Antibody in Immunocytochemistry (ICC) — Immunocytochemistry-Immunofluorescence analysis of C/EBP beta was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: C/EBP beta Polyclonal Antibody (Product # PA5 27244) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker.

C/EBP beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CEBP beta in paraffin-embedded ovarian carcinoma using a CEBP beta polyclonal antibody (Product # PA5-27244) at a dilution of 1:250.

C/EBP beta Antibody in Western Blot (WB) — Western blot was performed using Anti-C/EBP beta Polyclonal Antibody (Product # PA5-27244) and a band at ~36 kDa corresponding to C/EBP beta was observed across cell lines tested. Modified whole cell extracts (30 µg lysate) of A-431 (Lane 1), SK-OV-3 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

C/EBP beta Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of CEBPB protein from HeLa nuclear extracts using 5 µg of C/EBP beta antibody (Product # PA5-27244).

C/EBP beta Antibody in ChIP assay (ChIP) — Cross-linked ChIP was performed with Raji chromatin extract and 5 µg of either control rabbit IgG or a CEBP beta polyclonal antibody (Product # PA5-27244). The precipitated DNA was detected by PCR with primer set targeting to CR2pD.

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay-Dependent

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (IHC)

Assay Dependent

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 95 of C/EBP beta

Conjugate

Unconjugated

Form

Liquid

Concentration

0.37 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% Proclin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2544720

Product Specific Information

Recommended positive controls: U87-MG.

Predicted reactivity: Pig (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Cite this antibody

Bioinformatics

Protein Aliases: C EBP; C/EBP beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; interleukin 6-dependent DNA-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; Nuclear factor NF-IL6; nuclear factor of interleukin 6; OTTMUSP00000017503; TCF-5; Transcription factor 5; transcription factor C/EBP beta

Gene Aliases: C/EBP-beta; CEBPB; IL6DBP; NF-IL6; PP9092; TCF5

UniProt ID: (Human) P17676

Entrez Gene ID: (Human) 1051

Molecular Function: nucleic acid binding transcription factor