Invitrogen

C/EBP beta Polyclonal Antibody

Product Image Gallery

C/EBP beta Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of C/EBP beta was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: C/EBP beta Polyclonal Antibody (Product # PA5 27244) diluted at 1:1000. Red: phalloidin, a cytoskeleton marker.

C/EBP beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CEBP beta in paraffin-embedded ovarian carcinoma using a CEBP beta polyclonal antibody (Product # PA5-27244) at a dilution of 1:250.

C/EBP beta Antibody in Western Blot (WB) — Western blot was performed using Anti-C/EBP beta Polyclonal Antibody (Product # PA5-27244) and a band at ~36 kDa corresponding to C/EBP beta was observed across cell lines tested. Modified whole cell extracts (30 µg lysate) of A-431 (Lane 1), SK-OV-3 (Lane 2) and HeLa (Lane 3) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

C/EBP beta Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of CEBPB protein from HeLa nuclear extracts using 5 µg of C/EBP beta antibody (Product # PA5-27244).

C/EBP beta Antibody in ChIP assay (ChIP) — Cross-linked ChIP was performed with Raji chromatin extract and 5 µg of either control rabbit IgG or a CEBP beta polyclonal antibody (Product # PA5-27244). The precipitated DNA was detected by PCR with primer set targeting to CR2pD.

Product Details

PA5-27244

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (IHC)	Assay-dependent	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	1:100-1:500	-
ChIP assay (ChIP)	Assay-Dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide corresponding to a region within amino acids 1 and 95 of C/EBP beta View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.37 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol, 1% BSA
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2544720

Product Specific Information

Recommended positive controls: U87-MG.

Predicted reactivity: Pig (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of mesenchymal tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Metformin inhibition of mitochondrial ATP and DNA synthesis abrogates NLRP3 inflammasome activation and pulmonary inflammation.

Immunity

Xian H,Liu Y,Rundberg Nilsson A,Gatchalian R,Crother TR,Tourtellotte WG,Zhang Y,Aleman-Muench GR,Lewis G,Chen W,Kang S,Luevanos M,Trudler D,Lipton SA,Soroosh P,Teijaro J,de la Torre JC,Arditi M,Karin M,Sanchez-Lopez E

PA5-27244 was used in ChIP assay to conclude that inhibition of ATP and mtDNA synthesis is sufficient for ARDS amelioration.

Tue Jul 13 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: C EBP; C/EBP beta; CCAAT/enhancer binding protein (C/EBP), beta; CCAAT/enhancer-binding protein beta; interleukin 6-dependent DNA-binding protein; LAP; LIP; Liver activator protein; Liver-enriched inhibitory protein; Nuclear factor NF-IL6; nuclear factor of interleukin 6; OTTMUSP00000017503; TCF-5; Transcription factor 5; transcription factor C/EBP beta

Gene Aliases: C/EBP-beta; CEBPB; IL6DBP; NF-IL6; PP9092; TCF5

UniProt ID: (Human) P17676

Entrez Gene ID: (Human) 1051

Molecular Function: basic leucine zipper transcription factor