|Tested species reactivity||Rat|
|Host / Isotype||Chicken / IgY|
|Immunogen||Rat C-reactive protein peptide (aa 108-121)|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:2000 - 1:5000|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Western Blot (WB)||1:2000 - 1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-9579 detects CRP in rat samples. May cross react with mouse, human and other mammalia samples.
PA1-9579 has been successfully used in Western blot, ICC, IHC, and ELISA procedures. By Western blot, PA1-9579 detects a 23 kDa protein representing CRP.
PA1-9579 immunogen is a synthetic peptide corresponding to amino acids 108-121 of rat CRP.
C Reactive Protein is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is a pentraxin (cyclic pentameric protein) compound of five identical nonglycosylated subunits of 206 amino acids each (m.w. 24 kDa), that are bound noncovalently to form the physiologic CRP molecule (m.w. 117.5 kDa). C Reactive Protein mediates activities associated with preimmune nonspecific host resistance. It is opsonic, an initiator of the classical complement cascade and an activator of monocytes/macrophages. CRP also binds to several nuclear components including chromatin, histones and snRNP, suggesting that it may play a role as a scavenger during cell necrosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.