Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
C19orf10 Antibody (500-P259-1MG) in WB
Western Blot: To detect Murine SF-20 by Western Blot analysis C19orf10 Polyclonal Antibody (Product # 500-P259-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SF-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Product Details
500-P259-1MG
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived Recombinant Murine SF-20
Conjugate
Form
Lyophilized
Concentration
0.1-1.0 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient
RRID
AB_2930117
Product Specific Information
AA Sequence of recombinant protein: MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography employing an immobilized Murine SF-20 matrix.
Sandwich ELISA: To detect Murine SF-20 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine SF-20 (500-P259Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SF-20.
Western Blot: To detect Murine SF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SF-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Target Information
The interleukins are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. IL-25 (also called SF20/IL-25) is a secreted bone marrow stroma-derived growth factor cytokine that is derived from Th2 T-cells. IL-25 belongs to the IL-17 cytokine family and is capable of amplifying allergic type inflammatory responses by its actions on other cell types. IL-25 binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. Infusion of mice with IL-25 induces IL-4, IL-5, and IL-13 gene expression. IL-25 induced mice also develop epithelial cell hyperplasia, increased mucus secretion, and airway hyperreactivity, suggesting that IL-25 may be an important mediator of allergic disease via production of IL-4, IL-5, IL-13 and eotaxin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CS010; IL 25; IL 27; Interleukin25; MYDGF; Myeloid-derived growth factor; R33729 1; R337291; UPF0556 protein C19orf10 homolog
Gene Aliases: D17Wsu104e; Il25; Ly6elg; Mydgf
UniProt ID: (Mouse) Q9CPT4
Entrez Gene ID: (Mouse) 28106
angiogenesis
positive regulation of protein phosphorylation
positive regulation of endothelial cell proliferation
apoptotic process
positive regulation of phosphatidylinositol 3-kinase signaling
negative regulation of apoptotic process
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of transcription from RNA polymerase II promoter
positive regulation of protein kinase B signaling
cellular response to brefeldin A
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support