Immunofluorescent analysis of C1orf74 showing staining in the cytoplasm of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a C1orf74 polyclonal antibody (Product # PA5-31680) diluted at 1:1000. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 61 and 269 of Human C1orf74|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31680 targets C1orf74 in WB applications and shows reactivity with Human samples.
The PA5-31680 immunogen is recombinant fragment corresponding to a region within amino acids 61 and 269 of Human C1orf74.
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