|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||C4c complement isolated from complement activated human serum.|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 1 publications below|
PA1-28407 detects C4c / C4b from human samples.
PA1-28407 has been successfully used in immunofluorescence applications.
The PA1-28407 immunogen is C4c complement isolated from complement activated human serum.
This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain may be cleaved to release C4 anaphylatoxin, a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Commercially available complement component-depleted sera are unexpectedly codepleted of ficolin-2.
PA1-28407 was used in flow cytometry to study the presence or absence of ficolins in commercial complement component-depleted sera
|Brady AM,Geno KA,Dalecki AG,Cheng X,Nahm MH||Clinical and vaccine immunology : CVI (21:1323)||2014|