|Tested species reactivity||Mouse|
|Host / Isotype||Rat / IgG2b|
|Immunogen||RBL-2H3 transfected cells expressing murine C5aR.|
|Storage buffer||PBS with 1% BSA, 5% sucrose|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||Neat-1/10|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Clone 20/70 has been reported to block the binding of the C5a to murine CD88.
Reconstitute with 1 ml of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
CD88 is a 45kDa G-protein coupled cell surface receptor, also known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, which is a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.
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