Proteintech

C9orf72 Polyclonal Antibody

C9orf72 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 20

C9orf72 Antibody (22637-1-AP) in ICC/IF

Immunofluorescent analysis of SH-SY5Y cells using 22637-1-AP (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

C9orf72 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

C9orf72 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

22637-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 27 publications 27 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:2,000

Immunocytochemistry (ICC/IF)

1:10-1:100

View 8 publications 8 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C9orf72 Fusion Protein Ag18326 (1-169 aa encoded by BC020851)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.26 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MSTLCPPPS PAVAKTEIAL SGKSPLLAAT FAYWDNILGP RVRHIWAPKT EQVLLSDGEI TFLANHTLNG EILRNAESGA IDVKFFVLSE KGVIIVSLIF DGNWNGDRST YGLSIILPQT ELSFYLPLHR VCVDRLTHII RKGRIWMHKE RQENVQKIIL EGTERMEDQG (1-169 aa encoded by BC020851)

Target Information

C9orf72 may play a role in endosomal trafficking and autophagy. It is known to interact with HNRNPA1, HNRNPA2B1, UBQLN2, and small Rab GTPSases. C9orf72 localizes in the nucleus, cytoplasm, endosome, lysosome, and autophagosome. Mutations in this gene can result in frontotemporal dementia and/or amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Reversing lysosome-ribosome circuit dysregulation mitigates C9FTD/ALS neurodegeneration and behaviors.

Human molecular genetics

Ma L,Liang C,Wang J,Chang Q,Wang Y,Zhang W,Du Y,Sadan J,Chen JF

Thu Apr 06 00:00:00 UTC 2023

C9ORF72 suppresses JAK-STAT mediated inflammation.

iScience

Pang W,Hu F

Fri May 19 00:00:00 UTC 2023

C9orf72 functions in the nucleus to regulate DNA damage repair.

Cell death and differentiation

He L,Liang J,Chen C,Chen J,Shen Y,Sun S,Li L

Wed Mar 01 00:00:00 UTC 2023

C9orf72 knockdown alleviates hepatic insulin resistance by promoting lipophagy.

Biochemical and biophysical research communications

Cang X,Wang Y,Zeng J,Gao J,Yu Q,Lu C,Xu F,Lin J,Zhu J,Wang X

Sat Jan 15 00:00:00 UTC 2022

C9orf72 knockdown alleviates hepatic insulin resistance by promoting lipophagy.

Biochemical and biophysical research communications

Cang X,Wang Y,Zeng J,Gao J,Yu Q,Lu C,Xu F,Lin J,Zhu J,Wang X

Sat Jan 15 00:00:00 UTC 2022

C9orf72 knockdown alleviates hepatic insulin resistance by promoting lipophagy.

Biochemical and biophysical research communications

Cang X,Wang Y,Zeng J,Gao J,Yu Q,Lu C,Xu F,Lin J,Zhu J,Wang X

Sat Jan 15 00:00:00 UTC 2022

Staufen1 in Human Neurodegeneration.

Annals of neurology

Paul S,Dansithong W,Figueroa KP,Gandelman M,Scoles DR,Pulst SM

Tue Jun 01 00:00:00 UTC 2021

Expression of C9orf72 hexanucleotide repeat expansion leads to formation of RNA foci and dipeptide repeat proteins but does not influence autophagy or proteasomal function in neuronal cells.

Biochimica et biophysica acta. Molecular cell research

Leskelä S,Huber N,Hoffmann D,Rostalski H,Remes AM,Takalo M,Hiltunen M,Haapasalo A

Tue Jun 01 00:00:00 UTC 2021

Ablation of C9orf72 together with excitotoxicity induces ALS in rats.

The FEBS journal

Dong W,Ma Y,Guan F,Zhang X,Chen W,Zhang L,Zhang L

Mon Mar 01 00:00:00 UTC 2021

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Cell metabolism

Wang T,Liu H,Itoh K,Oh S,Zhao L,Murata D,Sesaki H,Hartung T,Na CH,Wang J

Tue Mar 02 00:00:00 UTC 2021

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Cell metabolism

Wang T,Liu H,Itoh K,Oh S,Zhao L,Murata D,Sesaki H,Hartung T,Na CH,Wang J

Tue Mar 02 00:00:00 UTC 2021

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Cell metabolism

Wang T,Liu H,Itoh K,Oh S,Zhao L,Murata D,Sesaki H,Hartung T,Na CH,Wang J

Tue Mar 02 00:00:00 UTC 2021

C9orf72 regulates energy homeostasis by stabilizing mitochondrial complex I assembly.

Cell metabolism

Wang T,Liu H,Itoh K,Oh S,Zhao L,Murata D,Sesaki H,Hartung T,Na CH,Wang J

Tue Mar 02 00:00:00 UTC 2021

G₄C₂ Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD.

Neuron

Coyne AN,Zaepfel BL,Hayes L,Fitchman B,Salzberg Y,Luo EC,Bowen K,Trost H,Aigner S,Rigo F,Yeo GW,Harel A,Svendsen CN,Sareen D,Rothstein JD

Wed Sep 23 00:00:00 UTC 2020

BV-2 Microglial Cells Overexpressing C9orf72 Hexanucleotide Repeat Expansion Produce DPR Proteins and Show Normal Functionality but No RNA Foci.

Frontiers in neurology

Rostalski H,Hietanen T,Leskelä S,Behánová A,Abdollahzadeh A,Wittrahm R,Mäkinen P,Huber N,Hoffmann D,Solje E,Remes AM,Natunen T,Takalo M,Tohka J,Hiltunen M,Haapasalo A

Sat Oct 31 00:00:00 UTC 2020

C9orf72/ALFA-1 controls TFEB/HLH-30-dependent metabolism through dynamic regulation of Rag GTPases.

PLoS genetics

Ji YJ,Ugolino J,Zhang T,Lu J,Kim D,Wang J

Wed Apr 01 00:00:00 UTC 2020

Analysis of ALS-related proteins during herpes simplex virus-2 latent infection.

Journal of neuroinflammation

Cabrera JR,Rodríguez-Izquierdo I,Jiménez JL,Muñoz-Fernández MÁ

Mon Dec 07 00:00:00 UTC 2020

Analysis of ALS-related proteins during herpes simplex virus-2 latent infection.

Journal of neuroinflammation

Cabrera JR,Rodríguez-Izquierdo I,Jiménez JL,Muñoz-Fernández MÁ

Mon Dec 07 00:00:00 UTC 2020

Reduced C9ORF72 function exacerbates gain of toxicity from ALS/FTD-causing repeat expansion in C9orf72.

Nature neuroscience

Zhu Q,Jiang J,Gendron TF,McAlonis-Downes M,Jiang L,Taylor A,Diaz Garcia S,Ghosh Dastidar S,Rodriguez MJ,King P,Zhang Y,La Spada AR,Xu H,Petrucelli L,Ravits J,Da Cruz S,Lagier-Tourenne C,Cleveland DW

Fri May 01 00:00:00 UTC 2020

C9orf72-associated SMCR8 protein binds in the ubiquitin pathway and with proteins linked with neurological disease.

Acta neuropathologica communications

Goodier JL,Soares AO,Pereira GC,DeVine LR,Sanchez L,Cole RN,García-Pérez JL

Thu Jul 16 00:00:00 UTC 2020

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

eLife

Laflamme C,McKeever PM,Kumar R,Schwartz J,Kolahdouzan M,Chen CX,You Z,Benaliouad F,Gileadi O,McBride HM,Durcan TM,Edwards AM,Healy LM,Robertson J,McPherson PS

Tue Oct 15 00:00:00 UTC 2019

Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.

eLife

Laflamme C,McKeever PM,Kumar R,Schwartz J,Kolahdouzan M,Chen CX,You Z,Benaliouad F,Gileadi O,McBride HM,Durcan TM,Edwards AM,Healy LM,Robertson J,McPherson PS

Tue Oct 15 00:00:00 UTC 2019

C9orf72 Proteins Regulate Autophagy and Undergo Autophagosomal or Proteasomal Degradation in a Cell Type-Dependent Manner.

Cells

Leskelä S,Huber N,Rostalski H,Natunen T,Remes AM,Takalo M,Hiltunen M,Haapasalo A

Thu Oct 10 00:00:00 UTC 2019

Disease models & mechanisms

Lynch E,Semrad T,Belsito VS,FitzGibbons C,Reilly M,Hayakawa K,Suzuki M

Fri Aug 16 00:00:00 UTC 2019

Disease models & mechanisms

Lynch E,Semrad T,Belsito VS,FitzGibbons C,Reilly M,Hayakawa K,Suzuki M

Fri Aug 16 00:00:00 UTC 2019

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Nature medicine

Shi Y,Lin S,Staats KA,Li Y,Chang WH,Hung ST,Hendricks E,Linares GR,Wang Y,Son EY,Wen X,Kisler K,Wilkinson B,Menendez L,Sugawara T,Woolwine P,Huang M,Cowan MJ,Ge B,Koutsodendris N,Sandor KP,Komberg J,Vangoor VR,Senthilkumar K,Hennes V,Seah C,Nelson AR,Cheng TY,Lee SJ,August PR,Chen JA,Wisniewski N,Hanson-Smith V,Belgard TG,Zhang A,Coba M,Grunseich C,Ward ME,van den Berg LH,Pasterkamp RJ,Trotti D,Zlokovic BV,Ichida JK

Thu Mar 01 00:00:00 UTC 2018

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Nature medicine

Thu Mar 01 00:00:00 UTC 2018

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Acta neuropathologica

Saberi S,Stauffer JE,Jiang J,Garcia SD,Taylor AE,Schulte D,Ohkubo T,Schloffman CL,Maldonado M,Baughn M,Rodriguez MJ,Pizzo D,Cleveland D,Ravits J

Thu Mar 01 00:00:00 UTC 2018

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Acta neuropathologica

Saberi S,Stauffer JE,Jiang J,Garcia SD,Taylor AE,Schulte D,Ohkubo T,Schloffman CL,Maldonado M,Baughn M,Rodriguez MJ,Pizzo D,Cleveland D,Ravits J

Thu Mar 01 00:00:00 UTC 2018

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 UTC 2018

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 UTC 2018

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 UTC 2018

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 UTC 2018

Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Amyotrophic lateral sclerosis & frontotemporal degeneration

Davidson YS,Robinson AC,Rollinson S,Pickering-Brown S,Xiao S,Robertson J,Mann DMA

Thu Feb 01 00:00:00 UTC 2018

Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Amyotrophic lateral sclerosis & frontotemporal degeneration

Davidson YS,Robinson AC,Rollinson S,Pickering-Brown S,Xiao S,Robertson J,Mann DMA

Thu Feb 01 00:00:00 UTC 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 UTC 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 UTC 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 UTC 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 UTC 2018

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 UTC 2017

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 UTC 2017

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 UTC 2017

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 UTC 2017

C9orf72 is required for proper macrophage and microglial function in mice.

Science (New York, N.Y.)

O'Rourke JG,Bogdanik L,Yáñez A,Lall D,Wolf AJ,Muhammad AK,Ho R,Carmona S,Vit JP,Zarrow J,Kim KJ,Bell S,Harms MB,Miller TM,Dangler CA,Underhill DM,Goodridge HS,Lutz CM,Baloh RH

Fri Mar 18 00:00:00 UTC 2016

C9orf72 is required for proper macrophage and microglial function in mice.

Science (New York, N.Y.)

O'Rourke JG,Bogdanik L,Yáñez A,Lall D,Wolf AJ,Muhammad AK,Ho R,Carmona S,Vit JP,Zarrow J,Kim KJ,Bell S,Harms MB,Miller TM,Dangler CA,Underhill DM,Goodridge HS,Lutz CM,Baloh RH

Fri Mar 18 00:00:00 UTC 2016

Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

Science translational medicine

Burberry A,Suzuki N,Wang JY,Moccia R,Mordes DA,Stewart MH,Suzuki-Uematsu S,Ghosh S,Singh A,Merkle FT,Koszka K,Li QZ,Zon L,Rossi DJ,Trowbridge JJ,Notarangelo LD,Eggan K

Wed Jul 13 00:00:00 UTC 2016

Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

Science translational medicine

Burberry A,Suzuki N,Wang JY,Moccia R,Mordes DA,Stewart MH,Suzuki-Uematsu S,Ghosh S,Singh A,Merkle FT,Koszka K,Li QZ,Zon L,Rossi DJ,Trowbridge JJ,Notarangelo LD,Eggan K

Wed Jul 13 00:00:00 UTC 2016

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

The EMBO journal

Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N

Wed Jun 15 00:00:00 UTC 2016

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

The EMBO journal

Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N

Wed Jun 15 00:00:00 UTC 2016

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 UTC 2016

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 UTC 2016

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 UTC 2016

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 UTC 2016

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Acta neuropathologica communications

Sullivan PM,Zhou X,Robins AM,Paushter DH,Kim D,Smolka MB,Hu F

Wed May 18 00:00:00 UTC 2016

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Acta neuropathologica communications

Sullivan PM,Zhou X,Robins AM,Paushter DH,Kim D,Smolka MB,Hu F

Wed May 18 00:00:00 UTC 2016

Bioinformatics

Protein Aliases: Guanine nucleotide exchange factor C9orf72; Guanine nucleotide exchange factor C9orf72 homolog; protein C9orf72 homolog

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Gene Aliases: AI840585; ALSFTD; C9orf72; DENND9; DENNL72; FTDALS; FTDALS1

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UniProt ID: (Mouse) Q6DFW0, (Human) Q96LT7, (Rat) Q66HC3

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Entrez Gene ID: (Mouse) 73205, (Human) 203228, (Rat) 313155

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Function(s)

protein binding Rab GTPase binding

Process(es)

endocytosis autophagy

Performance Guarantee

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C9orf72 Polyclonal Antibody

C9orf72 Antibody (22637-1-AP) in ICC/IF

Product Details

22637-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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