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C9orf72 Polyclonal Antibody

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C9orf72 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of SH-SY5Y cells using 22637-1-AP (C9orf72 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

C9orf72 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human gliomas tissue slide using 22637-1-AP ( C9orf72 antibody) at dilution of 1:1000 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

C9orf72 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 22637-1-AP (C9orf72 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

C9orf72 Antibody in Immunoprecipitation (IP) — IP result of anti-C9orf72 (IP: 22637-1-AP, 3 µg; Detection: 22637-1-AP 1:300) with SH-SY5Y cells lysate 2000 µg.

Product Details

22637-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 10 publications 10 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:10-1:100	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	C9orf72 Fusion Protein Ag18326 (1-169 aa encoded by BC020851) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.26 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSTLCPPPS PAVAKTEIAL SGKSPLLAAT FAYWDNILGP RVRHIWAPKT EQVLLSDGEI TFLANHTLNG EILRNAESGA IDVKFFVLSE KGVIIVSLIF DGNWNGDRST YGLSIILPQT ELSFYLPLHR VCVDRLTHII RKGRIWMHKE RQENVQKIIL EGTERMEDQG (1-169 aa encoded by BC020851)

Target Information

C9orf72 may play a role in endosomal trafficking and autophagy. It is known to interact with HNRNPA1, HNRNPA2B1, UBQLN2, and small Rab GTPSases. C9orf72 localizes in the nucleus, cytoplasm, endosome, lysosome, and autophagosome. Mutations in this gene can result in frontotemporal dementia and/or amyotrophic lateral sclerosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 EDT 2018

A C9orf72-CARM1 axis regulates lipid metabolism under glucose starvation-induced nutrient stress.

Genes & development

Liu Y,Wang T,Ji YJ,Johnson K,Liu H,Johnson K,Bailey S,Suk Y,Lu YN,Liu M,Wang J

Thu Nov 01 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

Novel antibodies reveal presynaptic localization of C9orf72 protein and reduced protein levels in C9orf72 mutation carriers.

Acta neuropathologica communications

Frick P,Sellier C,Mackenzie IRA,Cheng CY,Tahraoui-Bories J,Martinat C,Pasterkamp RJ,Prudlo J,Edbauer D,Oulad-Abdelghani M,Feederle R,Charlet-Berguerand N,Neumann M

Fri Aug 03 00:00:00 EDT 2018

Sense-encoded poly-GR dipeptide repeat proteins correlate to neurodegeneration and uniquely co-localize with TDP-43 in dendrites of repeat-expanded C9orf72 amyotrophic lateral sclerosis.

Acta neuropathologica

Saberi S,Stauffer JE,Jiang J,Garcia SD,Taylor AE,Schulte D,Ohkubo T,Schloffman CL,Maldonado M,Baughn M,Rodriguez MJ,Pizzo D,Cleveland D,Ravits J

Thu Mar 01 00:00:00 EST 2018

Haploinsufficiency leads to neurodegeneration in C9ORF72 ALS/FTD human induced motor neurons.

Nature medicine

Shi Y,Lin S,Staats KA,Li Y,Chang WH,Hung ST,Hendricks E,Linares GR,Wang Y,Son EY,Wen X,Kisler K,Wilkinson B,Menendez L,Sugawara T,Woolwine P,Huang M,Cowan MJ,Ge B,Koutsodendris N,Sandor KP,Komberg J,Vangoor VR,Senthilkumar K,Hennes V,Seah C,Nelson AR,Cheng TY,Lee SJ,August PR,Chen JA,Wisniewski N,Hanson-Smith V,Belgard TG,Zhang A,Coba M,Grunseich C,Ward ME,van den Berg LH,Pasterkamp RJ,Trotti D,Zlokovic BV,Ichida JK

Thu Mar 01 00:00:00 EST 2018

Immunohistochemical detection of C9orf72 protein in frontotemporal lobar degeneration and motor neurone disease: patterns of immunostaining and an evaluation of commercial antibodies.

Amyotrophic lateral sclerosis & frontotemporal degeneration

Davidson YS,Robinson AC,Rollinson S,Pickering-Brown S,Xiao S,Robertson J,Mann DMA

Thu Feb 01 00:00:00 EST 2018

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 EDT 2017

C9ORF72 Regulates Stress Granule Formation and Its Deficiency Impairs Stress Granule Assembly, Hypersensitizing Cells to Stress.

Molecular neurobiology

Maharjan N,Künzli C,Buthey K,Saxena S

Mon May 01 00:00:00 EDT 2017

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 EST 2016

Dynamic expression of the mouse orthologue of the human amyotropic lateral sclerosis associated gene C9orf72 during central nervous system development and neuronal differentiation.

Journal of anatomy

Ferguson R,Serafeimidou-Pouliou E,Subramanian V

Thu Dec 01 00:00:00 EST 2016

Loss-of-function mutations in the C9ORF72 mouse ortholog cause fatal autoimmune disease.

Science translational medicine

Burberry A,Suzuki N,Wang JY,Moccia R,Mordes DA,Stewart MH,Suzuki-Uematsu S,Ghosh S,Singh A,Merkle FT,Koszka K,Li QZ,Zon L,Rossi DJ,Trowbridge JJ,Notarangelo LD,Eggan K

Wed Jul 13 00:00:00 EDT 2016

Loss of C9ORF72 impairs autophagy and synergizes with polyQ Ataxin-2 to induce motor neuron dysfunction and cell death.

The EMBO journal

Sellier C,Campanari ML,Julie Corbier C,Gaucherot A,Kolb-Cheynel I,Oulad-Abdelghani M,Ruffenach F,Page A,Ciura S,Kabashi E,Charlet-Berguerand N

Wed Jun 15 00:00:00 EDT 2016

The ALS/FTLD associated protein C9orf72 associates with SMCR8 and WDR41 to regulate the autophagy-lysosome pathway.

Acta neuropathologica communications

Sullivan PM,Zhou X,Robins AM,Paushter DH,Kim D,Smolka MB,Hu F

Wed May 18 00:00:00 EDT 2016

C9orf72 is required for proper macrophage and microglial function in mice.

Science (New York, N.Y.)

O'Rourke JG,Bogdanik L,Yáñez A,Lall D,Wolf AJ,Muhammad AK,Ho R,Carmona S,Vit JP,Zarrow J,Kim KJ,Bell S,Harms MB,Miller TM,Dangler CA,Underhill DM,Goodridge HS,Lutz CM,Baloh RH