Immunofluorescent analysis of C9orf72 showing staining in the cytoplasm and nucleus of U87-MG cells. U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a C9orf72 polyclonal antibody (Product # PA5-31565) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 198 of Human C9orf72|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-31565 targets C9orf72 in IHC (P), ICC/IF, and WB applications and shows reactivity with Human and mouse samples.
The PA5-31565 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 198 of Human C9orf72.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.