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Immunofluorescence analysis of CA 125 was performed using 70% confluent log phase SKOV3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA 125 (M11) Mouse Monoclonal Antibody (Product # MA5-12425) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175)a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||CA 125 antigen derived from ascites fluid|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:40|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12425 targets CA 125 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12425 immunogen is cA 125 antigen derived from ascites fluid.
CA 125 determinant is present on a high molecular weight, mucin like glycoprotein of high molecular weight. CA 125 has been found on frozen sections of amnion and derivatives of coelomic and mullerian epithelium, including pleura, pericardium and peritoneum. In adult tissues, epithelial cells of fallopian tube, endometrium and endocervix; pancreas, colon, gall bladder, stomach, kidney, apocrine sweat gland and mammary gland. It is also found in mesothelial cell lining of pleura, pericardium and peritoneum. It is found in ovarian tumors of serous, endometrioid or clear cell types and adenocarcinomas of mullerian type.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A patient with a noninvasive mucinous ovarian borderline tumor presenting with late pleural metastases.
MA5-12425 was used in immunohistochemistry - paraffin section to describe a patient diagnosed with a noninvasive intestinal-type mucinous ovarian borderline tumor presenting with pleural metastases
|Simons M,Nagtegaal ID,Overbeek LI,Flucke U,Massuger LF,Bulten J||International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists (34:143)||2015|
Interfacial interaction between transmembrane ocular mucins and adhesive polymers and dendrimers analyzed by surface plasmon resonance.
MA5-12425 was used in western blot to develop a surface plasmon resonance method for studying interfacial interactions between ocular mucins and adhesion polymers
|Bravo-Osuna I,Noiray M,Briand E,Woodward AM,Argüeso P,Molina Martínez IT,Herrero-Vanrell R,Ponchel G||Pharmaceutical research (29:2329)||2012|
A metalloproteinase secreted by Streptococcus pneumoniae removes membrane mucin MUC16 from the epithelial glycocalyx barrier.
MA5-12425 was used in western blot to study the role of a Streptococcus pneumoniae secreted metalloprotease in breaching the epithelial glycocalyx barrier
|Govindarajan B,Menon BB,Spurr-Michaud S,Rastogi K,Gilmore MS,Argüeso P,Gipson IK||PloS one (7:null)||2012|
Gel-forming and cell-associated mucins: preparation for structural and functional studies.
MA5-12425 was used in western blot to survey methods for purifying gel-forming mucins associated with cell surface for structural and functional studies
|Davies JR,Wickström C,Thornton DJ||Methods in molecular biology (Clifton, N.J.) (842:27)||2012|
Notch signaling modulates MUC16 biosynthesis in an in vitro model of human corneal and conjunctival epithelial cell differentiation.
MA5-12425 was used in western blot to study the role of Notch signaling in modulating MUC16 biosynthesis in a model of human corneal and conjunctival epithelial cell differentiation
|Xiong L,Woodward AM,Argüeso P||Investigative ophthalmology & visual science (52:5641)||2011|
CA-125; CA125 ovarian cancer antigen; mucin-16; ovarian cancer-related tumor marker CA125; ovarian carcinoma antigen CA125