Immunofluorescence analysis of CA19-9 was performed using 70% confluent log phase SK-OV-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CA19-9 (116-NS-19-9) Mouse Monoclonal Antibody (MA5-12421) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human colon carcinoma cell line SW1116|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:20|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12421 targets CA19-9 in IHC (P) applications and shows reactivity with Human samples.
The MA5-12421 immunogen is human colon carcinoma cell line SW1116.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Pulmonary meningothelial-like nodules: new insights into a common but poorly understood entity.
MA5-12421 was used in immunohistochemistry to study pulmonary meningothelial-like nodules
|Mukhopadhyay S,El-Zammar OA,Katzenstein AL||The American journal of surgical pathology (33:487)||2009|
Introduction of Sd(a) carbohydrate antigen in gastrointestinal cancer cells eliminates selectin ligands and inhibits metastasis.
MA5-12421 was used in flow cytometry to study the loss of selectin ligands and reduced metastsis in human gastrointestinal tract cancer cells recombinantly expressing Sd(a) carbohydrate antigen
|Kawamura YI,Kawashima R,Fukunaga R,Hirai K,Toyama-Sorimachi N,Tokuhara M,Shimizu T,Dohi T||Cancer research (65:6220)||2005|