|Tested species reactivity||Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat CACNA1A.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||1:100|
|Western Blot (WB)||Assay-Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
For Western Blot, this antibody has non-specific bands at 42 kDa.
This antibody is predicted to react with rabbit, cow and human based on sequence homology.
Store antibody at 4°C for 1-2 weeks. For long-term storage, store at -20°C.
Voltage-dependent calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, and gene expression. Calcium channels are multisubunit complexes composed of alpha-1, beta, alpha-2/delta, and gamma subunits. The channel activity is directed by the pore-forming alpha-1 subunit, whereas, the others act as auxiliary subunits regulating this activity. The distinctive properties of the calcium channel types are related primarily to the expression of a variety of alpha-1 isoforms, alpha-1A, B, C, D, E, and S. This gene encodes the alpha-1A subunit, which is predominantly expressed in neuronal tissue. Mutations in this gene are associated with 2 neurologic disorders, familial hemiplegic migraine and episodic ataxia 2. This gene also exhibits polymorphic variation due to n-repeats. Multiple transcript variants encoding different isoforms have been found for this gene. In one set of transcript variants, the n-repeats occur in the 3' UTR, and are not associated with any disease. But in another set of variants, an insertion extends the coding region to include the n-repeats which encode a polyglutamine tract. Expansion of the n-repeats from the normal 4-16 to 21-28 in the coding region is associated with spinocerebellar ataxia 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Voltage-gated calcium channel autoimmune cerebellar degeneration: Case and study of cytotoxicity.
PA5-19598 was used in immunohistochemistry - paraffin section to characterize cytotoxicity through voltage-gated calcium channel autoimmune cerebellar degeneration
|McKasson M,Clardy SL,Clawson SA,Hill KE,Wood B,Carlson N,Bromberg M,Greenlee JE||Neurology(R) neuroimmunology and neuroinflammation (3:null)||2016|