MA3-921 detects 1,4-dihydropyridine (DHP) receptor alpha-2 subunit from human, rat, mouse, guinea pig and rabbit skeletal and cardiac muscle.
MA3-921 has been successfully used in Western blot, immunocytochemistry, immunofluorescence, and immunohistochemistry procedures. By Western blot, this antibody detects a 220 kDa protein under non-reducing and a 143 kDa protein under reducing conditions representing DHP from rabbit skeletal muscle membrane preparations. Immunohistochemical staining of DHP in rabbit skeletal muscle with MA3-921 results in double rows of discrete punctate staining representing pairs of triads on the opposing sides of the Z-lines.
The MA3-921 antigen is purified rabbit DHP receptor.
Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling.
The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
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Protein Aliases: Calcium Channel; Calcium channel subunit alpha 2 delta (dihydropyridine - sensitive L-type); calcium channel, L type, alpha 2 polypeptide; calcium channel, voltage-dependent, alpha 2/delta subunit 1; dihydropryridine-sensitive calcium channel alpha-2 subunit; dihydropyridine-sensitive calcium channel alpha 2; dihydropyridine-sensitive L-type, calcium channel alpha-2/delta subunit; Voltage-dependent calcium channel subunit alpha-2-1; Voltage-dependent calcium channel subunit alpha-2/delta-1; Voltage-dependent calcium channel subunit delta-1; Voltage-gated calcium channel subunit alpha-2/delta-1
Gene Aliases: Ca(v)alpha2delta1; CACNA2; CACNA2D1; CACNL2A; CCHL2A; CCHLA2; DHSCCA; LINC01112; lncRNA-N3; MHS3