|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:500|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 314-329 of mouse CAD|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-30545 detects CAD from mouse samples.
PA1-30545 has been successfully used in Western blot applications.
The PA1-30545 immunogen is: Synthetic peptide corresponding to amino acids 314-329 of mouse CAD
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2410008J01Rik; AU018859; CAD; Caspase-activated deoxyribonuclease; caspase-activated DNase; Cpad; DFF-40; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 kD, beta subunit; DNase inhibited by DNA fragmentation factor
Gene Aliases: 40kDa; 5730477D02Rik; CAD; CPAN; DFF40; Dffb; Didff
UniProt ID: (Mouse) O54788
Entrez Gene ID: (Mouse) 13368
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