Bethyl Laboratories

CAF-1 p60 Polyclonal Antibody

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CAF-1 p60 Antibody in Immunohistochemistry (IHC) — **Detection of human CAF-1 p60 by immunohistochemistry.** *Sample:* FFPE section of human small cell lung cancer. *Antibody:* Affinity purified rabbit anti-CAF-1 p60 (Cat. No. A301-085A) used at a dilution of 1:200 (1µg/ml). *Detection:* DAB.

CAF-1 p60 Antibody in Western Blot (WB) — **Detection of human CAF-1 p60 by western blot and immunoprecipitation.** *Samples:* Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 µg) cells. *Antibodies:* Affinity purified rabbit anti-CAF-1 p60 antibody A301-085A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CAF-1 p60 was also immunoprecipitated by rabbit anti-CAF-1 p60 antibody A301-084A, which recognizes an upstream epitope. *Detection:* Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).

Product Details

A301-085A

Applications	Tested Dilution	Publications
Western Blot (WB)	1:2,000-1:10,000	-
Immunohistochemistry (IHC)	1:100-1:500	-
Immunoprecipitation (IP)	2-5 µg/mg lysate	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Between 509 and C-term View immunogen
Conjugate	Unconjugated
Form	liquid
Concentration	0.20 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains	0.09% sodium azide
Storage conditions	4° C

Product Specific Information

The recommended shelf life for this product is 1 year from date of receipt.

Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Chromatin assembly factor 1 (CAF-1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF-1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF-1 are conserved among human, yeast, and Drosophila.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CAF-1 subunit B; CAF-I 60 kDa subunit; CAF-I p60; CAF1 p60; Chromatin assembly factor 1 subunit B; Chromatin assembly factor I p60 subunit; human chromatin assembly factor-I p60 subunit; M-phase phosphoprotein 7; p60 CAF1; p60-CAF1

Gene Aliases: CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60; CHAF1B; MPHOSPH7; MPP7

UniProt ID: (Human) Q13112

Entrez Gene ID: (Human) 8208

Molecular Function: chromatin/chromatin-binding, or -regulatory protein

Function(s)

positive regulation of defense response to virus by host DNA replication DNA repair DNA replication-dependent nucleosome assembly transcription, DNA-templated regulation of transcription, DNA-templated protein complex assembly cell cycle chromatin assembly mitophagy in response to mitochondrial depolarization xenophagy

Process(es)

chromatin binding histone binding unfolded protein binding

CAF-1 p60 Polyclonal Antibody

Product Details

A301-085A

Western Blot (WB)

Immunohistochemistry (IHC)

Immunoprecipitation (IP)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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