|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Mice were immunized with human CAF-1 p130 subunit protein and fusing the splenocytes with P3-X63-Ag8-653 mouse myeloma cells.|
|Storage buffer||1mg/ml BSA, 50% glycerol|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||2.5 µg/ml|
|Immunoprecipitation (IP)||2.5 µg|
|Western Blot (WB)||2.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-91703 detects CAF1A from human samples.
MA1-91703 has been successfully used in immunofluorescence, immunoprecipitation, Western blot applications.
The MA1-91703 immunogen is: Mice were immunized with human CAF-1 p130 subunit protein and fusing the splenocytes with P3-X63-Ag8-653 mouse myeloma cells.
Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.