|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||linear repetitive epitope in the PG region and allows for selective detection of both native and denatured Human CA IX.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:100-1:1000 (1x10^6 cells)|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Immunoprecipitation (IP)||2-10 µg/mg of lysate|
|Western Blot (WB)||1:100-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA5-16318 targets CAIX in FACS, IF, IHC-P, IP, and WB applications and shows reactivity with Human samples.
The MA5-16318 immunogen is linear repetitive epitope in the PG region and allows for selective detection of both native and denatured Human CA IX.
Carbonic anhydrase IX (CA IX) is a member of the CA family of zinc-binding enzymes that catalyze a reversible conversion between carbon dioxide and carbonic acid, in a reaction that involves facilitated hydration of CO2 to H2CO3 followed by the spontaneous dissociation of H2CO3 into bicarbonate and proton. The CA IX molecule consists of a large extracellular domain (ECD), single-pass transmembrane region (TM) and a short intracytoplasmic (IC) tail. The ECD contains an N-terminally located PG-like region (which is absent from the other carbonic anhydrase isoforms) and a centrally located, well conserved catalytic domain (CA). CA IX is a cell surface protein that is present in human tumors, but not in the corresponding normal tissues. Moreover, expression of CA IX correlates with poor prognosis in many tumor types. CA IX plays a role in two phenomena involved in development of tumor phenotype - control of cell adhesion and pH regulation. Tight association of CA IX with tumors is to a major part related to tumor hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Mineralization of bone-related SaOS-2 cells under physiological hypoxic conditions.
MA5-16318 was used in blocking or activating experiment and western blot to examine the metabolism of hypoxic SaOS-2 cells
|Müller WE,Schröder HC,Tolba E,Diehl-Seifert B,Wang X||The FEBS journal (283:74)||2016|