Immunofluorescent analysis of CALCOCO2 showing staining in the autophagosome of HeLa cells. HeLa cells mock (left) and treated with 50µM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CALCOCO2 monoclonal antibody (Product # MA5-18293) diluted at 1:1000. Red: Phalloidin, a F-actin marker.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 174 and 429 of NDP52|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles.
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