Immunofluorescent analysis of CAP43 was performed using 70% confluent log phase HeLa cells treated with 50ng of TNF-alpha for 20 minutes. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CAP43 Rabbit Polyclonal Antibody (42-6200) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e is untreated cell with less signal. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the N-terminal region of the human and predicted chimpanzee CAP43 proteins, which differs from predicted bovine by one amino acid change and from mouse and rat by two amino acid changes|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/mL|
|Immunofluorescence (IF)||2 µg/mL|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1-3 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NDRG1 is a 394 amino acid cytoplasmic protein belonging to the NDRG family. It interacts with APO A-I and A-II and plays a role in HDL-mediated cholesterol transport, stress responses, hormone responses, cell growth and differentiation. Its defect causes Charcot-Marie-Tooth disease type 4D (CMT4D) that is characterized by reduced nerve conduction velocities, segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes and hollow feet. NDRG1 plays a growth inhibitory role and serves as a marker of differentiation. It is expressed in placental membranes, prostate, kidney, small intestine, ovary tissues, colon and the cells that border the lumen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Prostate-derived ets factor represses tumorigenesis and modulates epithelial-to-mesenchymal transition in bladder carcinoma cells.
42-6200 was used in western blot to analyze repression of tumorigenesis and modulation of epithelial-to-mesenchymal transition in bladder carcinoma cells by prostate-derived ets factor
|Tsui KH,Lin YH,Chung LC,Chuang ST,Feng TH,Chiang KC,Chang PL,Yeh CJ,Juang HH||Cancer letters (375:142)||2016|
|Not Applicable||Not Cited||
Growth differentiation factor-15: a p53- and demethylation-upregulating gene represses cell proliferation, invasion, and tumorigenesis in bladder carcinoma cells.
42-6200 was used in western blot to analyze growth differentiation factor-15 as a p53- and demehtylation-upregulating gene that represses invasion, cell proliferation, and tumorigenesis in bladder carcinoma cells
|Tsui KH,Hsu SY,Chung LC,Lin YH,Feng TH,Lee TY,Chang PL,Juang HH||Scientific reports (5:null)||2015|
Metallothionein 3: an androgen-upregulated gene enhances cell invasion and tumorigenesis of prostate carcinoma cells.
42-6200 was used in western blot to study metallothioneins in prostate carcinoma cells.
|Juang HH,Chung LC,Sung HC,Feng TH,Lee YH,Chang PL,Tsui KH||The Prostate (73:1495)||2013|
Glycoprotein transmembrane nmb: an androgen-downregulated gene attenuates cell invasion and tumorigenesis in prostate carcinoma cells.
42-6200 was used in western blot to investigate the function and regulation of glycoprotein transmembrane nmb in the human prostate.
|Tsui KH,Chang YL,Feng TH,Chang PL,Juang HH||The Prostate (72:1431)||2012|
Upregulation of prostate-derived Ets factor by luteolin causes inhibition of cell proliferation and cell invasion in prostate carcinoma cells.
42-6200 was used in western blot to report the antitumor function of luteolin via upregulation of PDEF gene expression in human prostate carcinoma cells.
|Tsui KH,Chung LC,Feng TH,Chang PL,Juang HH||International journal of cancer (130:2812)||2012|
L-Mimosine blocks cell proliferation via upregulation of B-cell translocation gene 2 and N-myc downstream regulated gene 1 in prostate carcinoma cells.
42-6200 was used in western blot to investigate the molecular mechanisms of L-mimosine on cell cycle modulation in prostate carcinoma cells.
|Chung LC,Tsui KH,Feng TH,Lee SL,Chang PL,Juang HH||American journal of physiology. Cell physiology (302:C676)||2012|
N-myc downstream-regulated gene 1 (NDRG1) mediates pomegranate juice protection from apoptosis in hypoxic BeWo cells but not in primary human trophoblasts.
42-6200 was used in immunocytochemistry to test if pomegranate juice protects trophoblasts from hypoxia-induced apoptosis by modulating NDRG1 expression.
|Chen B,Zaveri PG,Longtine MS,Nelson DM||Placenta (36:847)||2015|
N-myc downstream-regulated gene 1 downregulates cell proliferation, invasiveness, and tumorigenesis in human oral squamous cell carcinoma.
42-6200 was used in immunocytochemistry and western blot to determine the role and function of NDRG1 in oral squamous cell carcinoma
|Lee JC,Chung LC,Chen YJ,Feng TH,Juang HH||Cancer letters (355:242)||2014|