Western blot analysis of CAPD3 in Hela cell lysates using a CAPD3 monoclonal antibody (Product # MA5-18070) at various dilutions. Samples were separated by SDS-PAGE and transferred to a nitrocellulose membrane. The membrane was blocked in 3% NFDM-PBST for 1 hr at room temperature and incubated at 4°C in 0.5% NFDM-PBST. Detection was performed using an HRP-conjugated Fc-specific anti-mouse secondary antibody.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||6xHis internal fragment of human CAP-D3|
|Contains||0.1% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:400|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This protein is a regulatory subunit of the condensin-2 complex, a complex which establishes mitotic chromosome architecture and is involved in physical rigidity of the chromatid axis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.