|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 27 and 253 of Human CBP80|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-30098 targets CBP80 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-30098 immunogen is recombinant fragment corresponding to a region within amino acids 27 and 253 of Human CBP80.
The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5' cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3'-end processing, RNA nuclear export, and nonsense-mediated mRNA decay.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
PA5-30098 was used in immunocytochemistry and western blot to elucidate the roles of nuclear cap-binding protein 1-3 in mRNA biogenesis
|Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A||Nature communications (6:null)||2015|