Invitrogen

CBS Monoclonal Antibody (GT519)

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

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Datasheet

Questions & Answers

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CBS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of CBS was performed in paraffin-embedded mouse kidney tissue using CBS Monoclonal Antibody (GT519) (Product # MA5-17273) at a dilution of 1:250.

CBS Antibody in Western Blot (WB) — Western blot was performed using Anti-CBS Monoclonal Antibody (GT519) (Product # MA5-17273) and a band at 61 kDa corresponding to CBS was observed across the cell lines and tissues tested. OVCAR-3 was observed to be high expressing cell line when compared to SK-OV-3; Mouse, Rat Liver and Mouse Pancreas were observed to be high expressing tissues when compared to Mouse and Rat Heart as reported in the literature. Whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), HEK-293 (Lane 2), HeLa (Lane 3), OVCAR-3 (Lane 4), SK-OV-3 (Lane 5), DU 145 (Lane 6), tissue extracts of Mouse Liver (Lane 7), Rat Liver (Lane 8), Mouse Pancreas (Lane 9), Mouse Heart (Lane 10) and Rat Heart (Lane 11) were electrophoresed using Novex® NuPAGE® 4-12% % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

CBS Antibody in Relative expression — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Relative expression of CBS was observed in OVCAR-3 when compared to SK-OV-3; Mouse, Rat Liver and Mouse Pancreas when compared to Mouse and Rat Heart using Anti-CBS Monoclonal Antibody (GT519) (Product # MA5-17273) in Western Blot. {RE}

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

GT519

Immunogen

Recombinant protein fragment contains a sequence corresponding to a region within amino acids 39 and 251 of CBS

Conjugate

Unconjugated

Form

Liquid

Concentration

0.97 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2538739

Product Specific Information

Recommended positive controls: HeLa, 293T, Raji, K562, mouse liver, rat liver.

Predicted reactivity: Mouse (94%), Rat (94%), Xenopus laevis (93%), Rabbit (95%), Bovine (94%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Beta-thionase; Cystathionine beta-synthase; Cystathionine beta-synthase-like protein; cystathionine-beta-synthase; Hemoprotein H-450; methylcysteine synthase; Serine sulfhydrase

Gene Aliases: AI047524; AI303044; CBS; CBSL; HIP4

UniProt ID: (Human) P0DN79, (Rat) P32232, (Mouse) Q91WT9

Entrez Gene ID: (Human) 875, (Rat) 24250, (Mouse) 12411

Molecular Function: deaminase dehydratase epimerase/racemase hydrolase isomerase lyase