Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescent analysis of CBX5 (green) in HeLa cells. The cells were fixed with formalin for 15 minutes, permeabilized with 1X Permeablilization buffer (Product # 8408400) for 15 minutes, and blocked with 1% Blocker BSA (Product #37525) for 15 minutes at room temperature. Cells were stained with CBX5 monoclonal antibody (Product # 730019) at a dilution of 1:250 in blocking buffer for one hour at room temperature, washed with 1X TBS Tween 20 Buffer (Product # 28360), followed by incubation with DyLight 488 goat anti-mouse IgG secondary antibody (green, Product # A28175) at a dilution of 1:2000 and Hoechst 33342 dye (blue, Product # 62249) at a dilution of 1:5000 for 30 minutes at room temperature. Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Human , Rat , Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||18-75 of 191 of CBX5 Protein.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg x 10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Immunoprecipitation (IP)||2 ug|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunoprecipitation (IP)||See 1 publications below|
730019 has successfully been used to detect CBX5 in mouse, human and rat.
730019 has been successfully used in western blotting and immunofluorescence to detect CBX5.
Heterochromatin protein-1 (HP1) is a methyl-lysine binding protein localized at heterochromatin sites, where it mediates gene silencing. It has been shown that mammalian methyltransferases that selectively methylate histone H3 on lysine-9 generate a binding site for HP1 proteins, a family of heterochromatic adaptor molecules implicated in both gene silencing and supranucleosomal chromatin structure. High-affinity in vitro recognition of a methylated histone H3 peptide by HP1 requires a functional chromodomain. Thus, the HP1 chromodomain is a specific interaction motif for the methyl epitope on lysine-9 of histone H3. In vivo, heterochromatin association of HP1 proteins is lost in Suv39h double-null primary mouse fibroblasts but is restored after reintroduction of a catalytically active SUV39H1 HMTase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Assessment of a method to characterize antibody selectivity and specificity for use in immunoprecipitation.
730019 was used in immunoprecipitation to describe a mass spectrometry-based method for scoring immunoprecipitation antibody quality
|Marcon E,Jain H,Bhattacharya A,Guo H,Phanse S,Pu S,Byram G,Collins BC,Dowdell E,Fenner M,Guo X,Hutchinson A,Kennedy JJ,Krastins B,Larsen B,Lin ZY,Lopez MF,Loppnau P,Miersch S,Nguyen T,Olsen JB,Paduch M,Ravichandran M,Seitova A,Vadali G,Vogelsang MS,Whiteaker JR,Zhong G,Zhong N,Zhao L,Aebersold R,Arrowsmith CH,Emili A,Frappier L,Gingras AC,Gstaiger M,Paulovich AG,Koide S,Kossiakoff AA,Sidhu SS,Wodak SJ,Gr?slund S,Greenblatt JF,Edwards AM||Nature methods (12:725)||2015|
Hp1alpha, Hp1a, HP1A, C75991, 2610029O15Rik, HP1
HP1 alpha, chromobox homolog 5, chromobox protein homolog 5, heterochromatin protein 1 alpha, heterochromatin protein 1 homolog alpha, mHP1(alpha), HP1 alpha homolog, HP1-ALPHA, HP1Hs alpha, antigen p25, chromobox homolog 5 (HP1 alpha homolog, Drosophila), heterochromatin protein 1-alpha