|Tested species reactivity||Dog, Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (76 GENNKPREKSESNKRK 91) of CBX5 protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||3-5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
CBX5, also known as Chromobox protein homolog 5, is a regulatory protein belonging to the Polycomb group proteins family with two chromo domains. CBX5 associates with chromosomes during mitosis, specifically with chromatin during metaphase and anaphase. A component of the centromeric and pericentromeric heterochromatin, CBX5 may interact with lamin B receptor (LBR) to contribute to the association of the heterochromatin with the inner nuclear membrane. CBX5 recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. This methyl-lysine binding protein localized at heterochromatin sites is also known to mediate gene silencing and is involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.