Immunofluorescence analysis of CCKAR was performed using 70% confluent log phase Panc-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CCKAR Rabbit Polyclonal Antibody (PA3116) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide, KLH conjugated, corresponding to the residues T(409) G A S L S R F S Y S H M S A S V P P Q (428) of human CCK1 receptor.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Western Blot (WB)||1:500 - 1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
PA3-116 detects CCK1-Receptor in human samples.
PA3-116 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects an ~80-90 kDa protein representing CCK1-Receptor in human samples. In immunohistochemistry, PAC1 immunoreactivity is predominantly localized at the plasma membrane and in cells of the gastric mucosa, myenteric ganglion cells, myenteric nerve fibers in the gasrtointestinal tract.
PA3-116 immunogen is a synthetic peptide, KLH conjugated, corresponding to the residues T(409) G A S L S R F S Y S H M S A S V P P Q (428) of human VPAC2 receptor.
The CCK1 receptor is a member of a family of G protein- coupled receptors that mediate the effects of cholecystokinin (CCK), a peptide hormone in the gastrointestinal tract. CCK1 receptors have been found to function in various tissues, including the gall bladder, gastric smooth muscle cells and mucosa, myenteric neurons, and the brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cholecystokinin receptor antagonist halts progression of pancreatic cancer precursor lesions and fibrosis in mice.
PA3-116 was used in immunohistochemistry - paraffin section to test if cholecystokinin contributes to the malignant progression of pancreatic intraepithelial neoplasia lesions and the fibrosis associated with pancreatic cancer
|Smith JP,Cooper TK,McGovern CO,Gilius EL,Zhong Q,Liao J,Molinolo AA,Gutkind JS,Matters GL||Pancreas (43:1050)||2014|