Immunofluorescence analysis of CCK2 cholecystokinin receptor 2 was performed using 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CCK2 cholecystokinin receptor 2 Rabbit Polyclonal Antibody (PA3-201) at 2 µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the C-terminal amino acids R(428)ALPDEDPPTPSIASLSRLSYTTISTLGPG(447) of human CCK2|
|Storage buffer||whole serum|
|Contains||0.02% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||1:5000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA3-201 detects CCK2 in human samples.
PA3-201 has successfully been used in Western blot and immunocytochemical procedures.
The PA3-201 immunogen is a synthetic peptide corresponding to the C-terminal amino acids R(428)ALPDEDPPTPSIASLSRLSYTTISTLGPG(447) of human CCK2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Immunohistochemical localization of CCK1 cholecystokinin receptors in normal and neoplastic human tissues.
PA3-201 was used in immunocytochemistry and western blot to investigate the expression of CCK1 receptor in human tissues
|Schulz S,Röcken C,Mawrin C,Schulz S||The Journal of clinical endocrinology and metabolism (90:6149)||2005|