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Immunofluorescent analysis of CCK4 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a CCK4 polyclonal antibody (Product # PA5-29839) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10µm.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 11 and 209 of Human CCK4|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29839 targets CCK4 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-29839 immunogen is recombinant protein fragment corresponding to a region within amino acids 11 and 209 of Human CCK4.
Receptor protein tyrosine kinases transduce extracellular signals across the cell membrane. A subgroup of these kinases lack detectable catalytic tyrosine kinase activity but retain roles in signal transduction. The protein encoded by this gene is a member of this subgroup of tyrosine kinases and may function as a cell adhesion molecule. This gene is thought to be expressed in colon carcinomas but not in normal colon, and therefore may be a marker for or may be involved in tumor progression. Four transcript variants encoding four different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
CCK-4; CCK4; colon carcinoma kinase 4; colon carcinoma kinase-4; inactive tyrosine-protein kinase 7; protein-tyrosine kinase 7; pseudo tyrosine kinase receptor 7; PTK7 protein tyrosine kinase 7; tyrosine-protein kinase-like 7
CCK-4; CCK4; PTK7