Immunohistochemistry was performed on acetone-fixed frozen rat testis sections. Tissues were blocked in 3% normal goat serum for 1 hour at room temperature and probed with a CCL2 polyclonal antibody (Product # PA1-22488) at a dilution of 1:500 for 1 hour and room temperature. Tissues were washed extensively with phosphate buffered saline (PBS). Detection was performed using a fluorophore-conjugated goat anti-rabbit IgG secondary antibody at a dilution of 1:500. Tissues were visualized by fluorescence microscopy and staining was observed in peritubular myoid cells. Inset = higher magnification. Data courtesy of the Innovators Program.
|Tested species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant, full length rat protein.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:1000|
|Western Blot (WB)||1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-22488 detects MCP1 in mouse and rat samples.
PA1-22488 has been successfully used in ICC/IF, IHC (F) and Western blot procedures.
The PA1-22488 immunogen is recombinant, full length rat protein.
Rat MCP-1/JE (monocyte chemoattractant protein-1) is a 148-amino acid CC chemokine with a NH2-terminal sequence of 29 residues as a signal sequence. It was originally cloned from Con A-stimulated rat spleen cDNA library. This rat MCP-1/JE is 49-amino acid longer than humanMCP-1 at 3'-end. This 3'-end is a serine and threonine rich zone, which is probably responsible for the extensive O-glycosylation and explains for the higher molecular weight (25 kDa). In vitro, MCP-1/JE is chemotactic for monocytes as well as lymphocytes and basophils, but not for neutrophils. MCP-1/JE is produced by a wide range of cell types as a reaction to diverse inflammatory stimuli.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.