Staining of 1-day LPS-stimulated human peripheral blood cells with 0.5 ug of Armenian Hamster IgG Isotype Control FITC (cat. 11-4888) (open histogram) or 0.5 ug of Anti-CCL2 (MCP-1) FITC (filled histogram). Cells in the monocyte gate were used for analysis.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Not Applicable|
|Host / Isotype||Armenian hamster / IgG|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Frozen) (IHC (F))||See 2 publications below|
Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.
Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. (Please use Functional Grade purified 2H5 antibody, cat. 16-7096, in functional assays.).
Applications Tested: This 2H5 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of LPS-stimulated normal human peripheral blood cells. This can be used at test size: 5 µL (0.5 µg)/test per test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||Mechanisms underlying renoprotection during renin-angiotensin system blockade.||Taal MW,Chertow GM,Rennke HG,Gurnani A,Jiang T,Shahsafaei A,Troy JL,Brenner BM,Mackenzie HS||American journal of physiology. Renal physiology (280:F343)||2001|
|Not Applicable||Not Cited||Macrophage inflammatory protein-2 and KC induce chemokine production by mouse astrocytes.||Luo Y,Fischer FR,Hancock WW,Dorf ME||Journal of immunology (Baltimore, Md. : 1950) (165:4015)||2000|