Normal human peripheral blood cells were stimulated with LPS for 5 hours in the presence of Monensin Solution (Product # 00-4505). The cells were intracellularly stained with Anti-Human CD11c PerCP-eFluor® 710 (Product # 46-0116) and 0.03 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714) (left) or 0.03 µg of Anti-Human CCL2 (MCP-1) APC (right) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824) and protocol. Cells in the monocyte gate were used for analysis.
|Tested species reactivity||Human|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Storage buffer||PBS, pH 7.2, with 0.1% gelatin|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, store in dark, DO NOT FREEZE!|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.06 µg/test|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
Description: The 5D3-F7 antibody reacts with human, cynomolgus, and rhesus monkey monocyte chemoattractant protein-1 (MCP-1), also known as monocyte chemotactic and activating factor (MCAF) and CCL2. MCP-1 is a CC chemokine which presents as a dimer. Derived from monocytes and vascular endothelial cells, it exerts chemoattractant activity on a variety of cell types, such as monocytes, basophils, T cells and NK cells via chemokine receptors such as CCR2, CCR5, and CCR10.
Applications Reported: This 5D3-F7 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 5D3-F7 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||The -2518 promotor polymorphism in the MCP-1 gene is associated with systemic sclerosis.||Karrer S,Bosserhoff AK,Weiderer P,Distler O,Landthaler M,Szeimies RM,Müller-Ladner U,Schölmerich J,Hellerbrand C||The Journal of investigative dermatology (124:92)||2005|
|Not Applicable||Not Cited||Human pancreatic islets produce and secrete MCP-1/CCL2: relevance in human islet transplantation.||Piemonti L,Leone BE,Nano R,Saccani A,Monti P,Maffi P,Bianchi G,Sica A,Peri G,Melzi R,Aldrighetti L,Secchi A,Di Carlo V,Allavena P,Bertuzzi F||Diabetes (51:55)||2002|