Invitrogen

CCL3 Monoclonal Antibody (93342), FITC

View all (28) CCL3 (MIP-1 alpha) antibodies

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CCL3 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of human peripheral blood monocytes treated with LPS were stained with mouse Anti-human CCL3/MIP-1a FITC-conjugated Monoclonal Antibody (Product # MA5-23564) or isotype control antibodyopen histogram. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µL/1x10^6 cells

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Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG2B

Class

Monoclonal

Type

Antibody

Clone

93342

Immunogen

E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92

Conjugate

FITC

Excitation/Emission Profile

Form

Liquid

Purification

Protein A/G

Storage buffer

Buffered saline solution with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_2609537

Target Information

Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; LD78-alpha(4-69); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; MIP-1-alpha(4-69); mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein

Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

UniProt ID: (Human) P10147

Entrez Gene ID: (Human) 6348

Molecular Function: chemokine cytokine signaling molecule

MAPK cascade osteoblast differentiation cell activation monocyte chemotaxis calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response cytoskeleton organization G-protein coupled receptor signaling pathway cell-cell signaling behavior regulation of cell shape response to toxic substance positive regulation of gene expression negative regulation of gene expression T cell chemotaxis release of sequestered calcium ion into cytosol by sarcoplasmic reticulum calcium-mediated signaling signaling positive regulation of cell migration negative regulation of bone mineralization neutrophil chemotaxis lipopolysaccharide-mediated signaling pathway positive regulation of tumor necrosis factor production eosinophil degranulation protein kinase B signaling positive regulation of neuron apoptotic process positive regulation of GTPase activity astrocyte cell migration negative regulation by host of viral transcription negative regulation of osteoclast differentiation eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of interleukin-1 beta secretion positive regulation of inflammatory response positive regulation of calcium-mediated signaling positive chemotaxis positive regulation of protein kinase B signaling positive regulation of calcium ion transport regulation of sensory perception of pain chemokine-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to organic cyclic compound granulocyte chemotaxis positive regulation of calcium ion import positive regulation of natural killer cell chemotaxis

Process(es)

protein kinase activity calcium-dependent protein kinase C activity protein binding chemokine activity phospholipase activator activity kinase activity CCR1 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity identical protein binding