Invitrogen

CCL3 Monoclonal Antibody (93342), FITC

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CCL3 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of human peripheral blood monocytes treated with LPS were stained with mouse Anti-human CCL3/MIP-1a FITC-conjugated Monoclonal Antibody (Product # MA5-23564) or isotype control antibodyopen histogram. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I.

Product Details

MA5-23564

Applications	Tested Dilution	Publications
Flow Cytometry (Flow)	10 µL/1x10^6 cells	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Mouse / IgG2B
Class	Monoclonal
Type	Antibody
Clone	93342
Immunogen	E. coli-derived recombinant human CCL3/MIP-1 alpha Ala27-Ala92 View antigen
Conjugate	FITC
Excitation/Emission Profile	View spectra
Form	Liquid
Purification	Protein A/G
Storage buffer	Buffered saline solution with BSA
Contains	0.09% sodium azide
Storage conditions	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2609537

Target Information

Macrophage inflammatory protein-1a (MIP-1a) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1a has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

CD28 Homolog Is a Strong Activator of Natural Killer Cells for Lysis of B7H7⁺ Tumor Cells.

Cancer immunology research

Zhuang X,Long EO

MA5-23564 was used in Flow cytometry/Cell sorting to show that CD28 homolog is expressed in primary natural killer cells and that it is a strong activator of natural killer cells through selective synergy with receptors NKp46 and 2B4 to induce degranulation, lysis of target cells, and production of proinflammatory cytokines.

Sat Jun 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; H-MIP-1-alpha; LD78-alpha; LD78-alpha(4-69); Macrophage inflammatory protein 1-alpha; MIP-1-alpha; MIP-1-alpha(4-69); mip1 alpha; PAT 464.1; RP23-320E6.7; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein

Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3

UniProt ID: (Human) P10147

Entrez Gene ID: (Human) 6348

Molecular Function: cytokine intercellular signal molecule