Invitrogen

CCL5 (RANTES) Recombinant Rabbit Monoclonal Antibody (25H14L17)

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CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RANTES showing staining in the cytoplasm of paraffin-embedded mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a RANTES Recombinant Rabbit Monoclonal Antibody (Product # 701030) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

CCL5 (RANTES) Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (1 µg) of Rat RANTES (Lane 1), Mouse RANTES (Lane 2) and Human RANTES (lane 3). The blots were probed with Anti-RANTES Recombinant Rabbit Monoclonal Antibody (Product # 701030, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 8 kDa band corresponding to RANTES was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

CCL5 (RANTES) Antibody in Western Blot (WB) — Western blot analysis of recombinant mouse RANTES (1 ng, lane1) and recombinant rat Rantes (1 ng, lane 2) using a RANTES recombinant rabbit monoclonal antibody (Product # 701030) at a dilution of 1 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).

Product Details

Tested Applications

Dilution

Immunohistochemistry (Paraffin) (IHC (P))

1:10-1:50

Western Blot (WB)

0.1-5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Recombinant Monoclonal

Type

Antibody

Clone

25H14L17

Immunogen

proprietary

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2532353

Product Specific Information

This antibody is predicted to react with human and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Rantes gene is one of CC cytokine genes which are present on chromosome 17 clustered on q arm and is associated with the inflammatory response. Rantes functions as a chemoattractant for blood monocytes, eosinophils, and memory T helper cells. Rantes shows inhibition of replication/entry of various strains of HIV and SIV.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; D17S136E; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; RANTES; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; SCYA5; SIS-delta; SISd; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

Gene Aliases: CCL5; D17S136E; eoCP; MuRantes; RANTES; SCYA5; SIS-delta; SISd; TCP228

UniProt ID: (Human) P13501, (Mouse) P30882

Entrez Gene ID: (Human) 6352, (Rat) 81780, (Mouse) 20304

Molecular Function: chemokine cytokine signaling molecule

MAPK cascade dendritic cell chemotaxis chronic inflammatory response monocyte chemotaxis regulation of chronic inflammatory response calcium ion transport cellular calcium ion homeostasis exocytosis chemotaxis inflammatory response leukocyte cell-cell adhesion G-protein coupled receptor signaling pathway cell-cell signaling aging response to virus response to toxic substance response to salt stress positive regulation of activation of JAK2 kinase activity positive regulation of macrophage chemotaxis positive regulation of T cell chemotaxis positive regulation of phosphatidylinositol 3-kinase signaling response to activity positive regulation of smooth muscle cell migration dibenzo-p-dioxin metabolic process positive regulation of cell migration neutrophil chemotaxis positive regulation of cellular biosynthetic process activation of phospholipase D activity positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to insulin positive regulation of cell-cell adhesion mediated by integrin positive regulation of homotypic cell-cell adhesion positive regulation of T cell proliferation neutrophil activation positive regulation of phosphorylation response to drug positive regulation of tyrosine phosphorylation of STAT protein protein kinase B signaling positive regulation of GTPase activity cellular protein complex assembly response to estrogen negative regulation by host of viral transcription cellular response to fibroblast growth factor stimulus positive regulation of viral genome replication negative regulation of viral genome replication positive regulation of innate immune response positive regulation of neuron differentiation positive regulation of osteoclast differentiation negative regulation of G-protein coupled receptor protein signaling pathway positive regulation of angiogenesis positive regulation of cell adhesion positive regulation of translational initiation positive regulation of JAK-STAT cascade eosinophil chemotaxis macrophage chemotaxis lymphocyte chemotaxis positive regulation of smooth muscle cell proliferation positive regulation of epithelial cell proliferation positive regulation of inflammatory response regulation of insulin secretion regulation of T cell activation positive chemotaxis protein tetramerization response to glucocorticoid positive regulation of calcium ion transport positive regulation of mast cell chemotaxis positive regulation of protein tyrosine kinase activity chemokine-mediated signaling pathway negative regulation of chemokine-mediated signaling pathway negative regulation of T cell apoptotic process positive regulation of T cell apoptotic process positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to amino acid stimulus cellular response to vitamin K cellular response to morphine cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to ethanol cellular response to high density lipoprotein particle stimulus cellular response to organic cyclic compound cellular response to alkyl hydroperoxide cellular response to transforming growth factor beta stimulus positive regulation of monocyte chemotaxis regulation of neuron death negative regulation of neuron death negative regulation of macrophage apoptotic process positive regulation of T cell migration positive regulation of natural killer cell chemotaxis protein phosphorylation response to bacterium positive regulation of gene expression response to organic cyclic compound pseudopodium assembly response to nutrient levels response to lipopolysaccharide response to cytokine response to interferon-gamma response to tumor necrosis factor T-helper 1 cell diapedesis negative regulation of cell death cellular response to lipopolysaccharide cellular response to cytokine stimulus cellular response to lipid positive regulation of defense response to virus by host immune response extracellular negative regulation of signal transduction negative regulation of receptor activity positive regulation of chemokine (C-X-C motif) ligand 2 production

Process(es)

phosphatidylinositol phospholipase C activity protein kinase activity protein binding chemokine activity heparin binding phospholipase activator activity receptor signaling protein tyrosine kinase activator activity CCR1 chemokine receptor binding CCR4 chemokine receptor binding CCR5 chemokine receptor binding chemoattractant activity chemokine receptor binding protein homodimerization activity protein self-association chemokine receptor antagonist activity cytokine activity