Invitrogen

CCL5 (RANTES) Recombinant Rabbit Monoclonal Antibody (25H14L17)

Product Image Gallery

CCL5 (RANTES) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of RANTES showing staining in the cytoplasm of paraffin-embedded mouse lung tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a RANTES Recombinant Rabbit Monoclonal Antibody (Product # 701030) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

CCL5 (RANTES) Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (1 µg) of Rat RANTES (Lane 1), Mouse RANTES (Lane 2) and Human RANTES (lane 3). The blots were probed with Anti-RANTES Recombinant Rabbit Monoclonal Antibody (Product # 701030, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 8 kDa band corresponding to RANTES was observed. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Product Details

701030

Applications	Tested Dilution	Publications
Western Blot (WB)	0.1-5 µg/mL	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:10-1:50	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	25H14L17
Immunogen	proprietary
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2532353

Product Specific Information

This antibody is predicted to react with human and rat based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

RANTES is a chemoattractant for blood monocytes, memory T helper cells and eosinophils. It causes the release of histamine from basophils and activates eosinophils. It binds to CCR1, CCR3, CCR4 and CCR5 and is one of the major HIV suppressive factors produced by CD8+ T cells. Recombinant RANTES protein induces a dose dependent inhibition of different strains of HIV 1, HIV 2, and simian immunodeficiency virus (SIV). It is T cell and macrophage specific, induced by mitogens, and belongs to the intercrine beta (chemokine CC) family.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Four-Chemokine Signature Is Associated with a T-cell-Inflamed Phenotype in Primary and Metastatic Pancreatic Cancer.

Clinical cancer research : an official journal of the American Association for Cancer Research

Romero JM,Grünwald B,Jang GH,Bavi PP,Jhaveri A,Masoomian M,Fischer SE,Zhang A,Denroche RE,Lungu IM,De Luca A,Bartlett JMS,Xu J,Li N,Dhaliwal S,Liang SB,Chadwick D,Vyas F,Bronsert P,Khokha R,McGaha TL,Notta F,Ohashi PS,Done SJ,O'Kane GM,Wilson JM,Knox JJ,Connor A,Wang Y,Zogopoulos G,Gallinger S

701030 was used in Immunohistochemistry to elucidate the molecular drivers of antitumor immunity in pancreatic ductal adenocarcinoma.

Wed Apr 15 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; MuRantes; OTTHUMP00000197106; RANTES(3-68); RANTES(4-68); regulated upon activation normal T-cell expressed and secreted; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine A5; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

Gene Aliases: CCL5; D17S136E; eoCP; MuRantes; RANTES; SCYA5; SIS-delta; SISd; TCP228

UniProt ID: (Human) P13501, (Mouse) P30882

Entrez Gene ID: (Human) 6352, (Rat) 81780, (Mouse) 20304

Molecular Function: cytokine intercellular signal molecule