Spectral confocal microscopy of CHO cells using MA1-17770. CHO cells were transiently transfected with an expression vector encoding CCR4. Binding of MA1-17770 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with Alexa Fluor-555 Phalloidin (red). Cell nuclei are stained with DAPI (blue).
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Synthetic peptide corresponding to human CCR4.|
|Storage buffer||PBS, pH 7.2|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1.2 µg/10^6 cells|
|Immunocytochemistry (ICC)||1 ug/10^6 cells|
|Immunofluorescence (IF)||1 µg/10^6 cells|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody recognizes native human CCR4 protein expressed in transfected mammalian cells.
Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.