|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Human CCR6 transfected NSO mouse myeloma cells.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5-10 µg for 10^5 cells in 100 µl|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-20156 detects CCR6 in human samples.
MA1-20156 has been successfully used in FACS and immunohistochemistry (frozen) procedures.
The MA1-20156 immunogen is human CCR6 transfected NSO mouse myeloma cells.
Endotoxin level is less than 10 ng per mg antibody as determined by the LAL method.
Chemokines have been sub-divided into families on the basis of the relative position of their cysteine residues. The a- and b- families, with four cysteine residues, are the largest and best characterized. CCR-6 (previously referred to as the GPR-CY4, CKRL3, or STRL22 orphan receptor) binds MIP-3". It is a CC chemokine receptor that is highly expressed in human dendritic cells derived from CD34+ cord blood precursors.2-4 In addition to dendritic cells, CCR-6 mRNA has also been detected in lymphocytes (CD4 and CD8 T cells and B cells), but not in natural killer cells, monocytes and granulocytes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.