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Western blot analysis of CCR7 was performed by loading the indicated amounts of Jurkat whole cell lysate and 10ul of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% nonfat dry milk in TBST for at least 1 hour at room temperature. CCR7 was detected at ~43kD using a CCR7 monoclonal antibody (Product # MA1-163) at a dilution of 1:500 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rat IgG secondary antibody (Product # 31470) at a dilution of 1:40,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34075).
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Rat basophilic leukaemia (RBL)-2H3 transfectants expressing mouse CCR7|
|Storage buffer||PBS with 1mg/ml BSA, 30% glycerol|
|Contains||0.05% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||5ug/1X10^6 cells|
|Western Blot (WB)||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-163 has been validated for flow cytometry on primary mouse splenocytes. Optimal staining is observed when incubating the cells with antibody at 37C for at least 30 minutes.
MA1-163 has been validated for Western blot on Jurkat cell lysates.
CCR7 is a member of the G protein coupled receptor family (subfamily: chemokine). This receptor was identified as a gene induced by the Epstein Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. CCR7 has been reported to be expressed in blood, bone marrow, lymph node, and intestine. It is particularly expressed in lymphoid tissues and in activated B and T lymphocytes and has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. ESTs have been isolated from blood, embryo, lymph node, and thymus libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bukitt's lymphoma receptor 2; C-C chemokine receptor type 7; CC chemokine receptor 7; CCR-7; CD197; Chemokine (C-C Motif) Receptor 7; chemokine (C-C) receptor 7; CMKBR7; EBI1; EBV-induced G protein-coupled receptor 1; EBV-induced G-protein coupled receptor 1; Epstein-Barr virus induced gene 1; Epstein-Barr Virus-Induced G-Protein Coupled Receptor 1; lymphocyte-specific G protein-coupled peptide receptor; MIP-3 Beta Receptor
BLR2; CC-CKR-7; CCR-7; CCR7; CD197; CDw197; CMKBR7; EBI1; Ebi1h; EVI1