|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Goat / IgG|
|Immunogen||Synthetic peptide corresponding to residues I(10) P G M F D D F S Y D S T A S T D D Y M N L N F S S F F(37) of mouse CCR9.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.01M potassium phosphate with 0.14M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA1-21618 detects CCR9 in mouse and human samples.
PA1-21618 has been successfully used in ELISA, FACS, immunocytochemistry, immunohistochemistry (paraffin) and Western blot procedures.
The PA1-21618 immunogen is a synthetic peptide corresponding to residues I(10) P G M F D D F S Y D S T A S T D D Y M N L N F S S F F(37) of mouse CCR9.
The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to the chemokine receptor gene cluster region. Two alternatively spliced transcript variants have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Comprehensive models of human primary and metastatic colorectal tumors in immunodeficient and immunocompetent mice by chemokine targeting.
PA1-21618 was used in western blot to describe the use of CCR9 expression to create primary gastrointestinal tumors in immunodeficient mice by tail-vein injection.
|Chen HJ,Sun J,Huang Z,Hou H,Arcilla M,Rakhilin N,Joe DJ,Choi J,Gadamsetty P,Milsom J,Nandakumar G,Longman R,Zhou XK,Edwards R,Chen J,Chen KY,Bu P,Wang L,Xu Y,Munroe R,Abratte C,Miller AD,Gümü¿ ZH,Shuler M,Nishimura N,Edelmann W,Shen X,Lipkin SM||Nature biotechnology (33:656)||2015|