Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm and membrane of Hela cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 (Product # PA5-16895) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Human|
|Published species reactivity||Human, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 26-176 of human CD105|
|Storage buffer||PBS, pH 7.6, with 0.2% BSA|
|Contains||15mM sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:25-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-16895 targets CD106 in IHC (P) and ICC/IF applications and shows reactivity with Human samples.
The PA5-16895 immunogen is recombinant protein encoding aa 26-176 of human CD105.
CD105 or Endoglin or GP160 is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker. CD105 binds to TGF-beta1 and beta3 with high affinity but not TGF-beta2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Deletion of IL-33R attenuates VEGF expression and enhances necrosis in mammary carcinoma.
PA5-16895 was used in immunohistochemistry - paraffin section to study the attenuation of VEGF expression and enhancement of necrosis in mammary carcinoma by deletion of IL-33R
|Milosavljevic MZ,Jovanovic IP,Pejnovic NN,Mitrovic SL,Arsenijevic NN,Simovic Markovic BJ,Lukic ML||Oncotarget (7:18106)||2016|
|Not Applicable||Not Cited||
Inhibition of hypoxia inducible factor 1 and topoisomerase with acriflavine sensitizes perihilar cholangiocarcinomas to photodynamic therapy.
PA5-16895 was used in immunohistochemistry to analyze inhibition of hypoxia inducible factor 1 and acriflavine in sensitizing perihilar cholagiocarcinomas to photodynamic therapy
|Weijer R,Broekgaarden M,Krekorian M,Alles LK,van Wijk AC,Mackaaij C,Verheij J,van der Wal AC,van Gulik TM,Storm G,Heger M||Oncotarget (7:3341)||2016|
Early onset of endothelial cell proliferation in coronary thrombi of patients with an acute myocardial infarction: implications for plaque healing.
PA5-16895 was used in immunohistochemistry to study neovascularization and the timing of endothelial cell proliferation in coronary thrombi following acute myocardial infarction
|Li X,Kramer MC,VAN DER Loos CM,Ploegmakers HJ,DE Boer OJ,Koch KT,Tijssen JG,DE Winter RJ,VAN DER Wal AC||Journal of thrombosis and haemostasis : JTH (10:466)||2012|