Immunofluorescent analysis of CD105/endoglin (green) showing staining in the in the membrane of human uterus tissue (right) compared to a negative control without primary antibody (left). Formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Tissue was probed with a CD105/endoglin monoclonal antibody (Product # MA5-11854) in 3% BSA-PBS at a dilution of 1:50 and incubated overnight at 4°C in a humidified chamber. Tissue was washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
|Tested species reactivity||Bovine, Human|
|Published species reactivity||Rabbit, Pig, Human, Mouse, Not Applicable|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||0.5-1 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100 - 200|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Flow Cytometry (Flow)||See 4 publications below|
|Immunohistochemistry (IHC)||See 10 publications below|
|Immunocytochemistry (ICC)||See 5 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
MA5-11854 targets CD105 in FACS, IF, IHC (P), and WB applications and shows reactivity with Human and Bovine samples.
The MA5-11854 immunogen is purified GP160 from cell membrane glycoproteins of fresh non-T/non-B acute lymphoblastic leukemia cells.
MA5-11854 was used in the immunofluorescence analysis to successfully detect CD105 in human mesenchymal stem cells derived from iPSCs.
CD105 or Endoglin or GP160 is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists as an O- and N-glycosylated homodimer. Up-regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation-associated endothelial cell marker. CD105 binds to TGF-Beta1 and Beta3 with high affinity but not TGF-Beta2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Genetically modified human placenta¿derived mesenchymal stem cells with FGF¿2 and PDGF¿BB enhance neovascularization in a model of hindlimb ischemia.
MA5-11854 was used in flow cytometry to study enhacement of neovascularization in a model of hindlimb ischemia by genetically modified human placenta-derived mesenchymal stem cells with PDGF-BB and FGF-2
|Yin T,He S,Su C,Chen X,Zhang D,Wan Y,Ye T,Shen G,Wang Y,Shi H,Yang L,Wei Y||Molecular medicine reports (12:5093)||2015|
Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation.
MA5-11854 was used in flow cytometry to investigate the regulatory role of estrogen signaling pathways in the differentiation of mesenchymal stem cells in vitro
|Zhao JW,Gao ZL,Mei H,Li YL,Wang Y||The American journal of the medical sciences (341:460)||2011|
Engineering an antiplatelet adhesion layer on an electrospun scaffold using porcine endothelial progenitor cells.
MA5-11854 was used in flow cytometry to evaluate an electrospun scaffold for the establishment of antiplatelet adhesion layer
|Zhang X,Xu Y,Thomas V,Bellis SL,Vohra YK||Journal of biomedical materials research. Part A (97:145)||2011|
[Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation].
MA5-11854 was used in flow cytometry to evaluate the utility of enhanced green fluorescent protein transduction in human mesenchymal stem cells
|He X,Li YL,Wang XR,Guo X,Niu Y||Chinese medical journal (118:1728)||2005|
Angiogenesis and expression of PDGF-C, VEGF, CD105 and HIF-1¿ in human glioblastoma.
MA5-11854 was used in immunohistochemistry to study the immunohistochemical expression of PDGF-C, VEGF, CD105 and HIK-1alpha and the implications for anti-angiogenic therapy in human glioblastoma
|Clara CA,Marie SK,de Almeida JR,Wakamatsu A,Oba-Shinjo SM,Uno M,Neville M,Rosemberg S||Neuropathology : official journal of the Japanese Society of Neuropathology (34:343)||2014|
Intravascular papillary endothelial hyperplasia: histomorphological and immunohistochemical features.
MA5-11854 was used in immunohistochemistry to study the morpholgy, histology and immunohistochemistry of intravascular papillary endothelial hyperplasia
|Akdur NC,Donmez M,Gozel S,Ustun H,Hucumenoglu S||Diagnostic pathology (8:null)||2013|
Mesenchymal-to-endothelial transition in Kaposi sarcoma: a histogenetic hypothesis based on a case series and literature review.
MA5-11854 was used in immunohistochemistry to develop a hypothesis of Kaposi sarcoma histogenesis based on a case and literature review
|Gurzu S,Ciortea D,Munteanu T,Kezdi-Zaharia I,Jung I||PloS one (8:null)||2013|
The differences between the endothelial area marked with CD31 and CD105 in colorectal carcinomas by computer-assisted morphometrical analysis.
MA5-11854 was used in immunohistochemistry to compare the CD31 and CD105 endothelial areas in colorectal carcinomas by computer-assisted morphometrical analysis
|Jung I,Gurzu S,Raica M,Cîmpean AM,Szentirmay Z||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (50:239)||2009|
The expression of cytoskeleton regulatory protein Mena in colorectal lesions.
MA5-11854 was used in immunohistochemistry to investigate the expression of mammalian actin regulatory protein Ena in colorectal lesions
|Gurzu S,Jung I,Prantner I,Ember I,Pávai Z,Mezei T||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (49:345)||2008|
The angiogenesis in colorectal carcinomas with and without lymph node metastases.
MA5-11854 was used in immunohistochemistry to study the prognostic value of angiogenesis in colorectal carcinomas with and without lymph node metastases
|Gurzu S,Jung J,Azamfirei L,Mezei T,Cîmpean AM,Szentirmay Z||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (49:149)||2008|
The aspects of angiogenesis in anal canal carcinomas compared with that in colorectal carcinomas.
MA5-11854 was used in immunohistochemistry to compare the aspects of angiogenesis between anal canal and colorectal carcinomas
|Gurzu S,Jung J,Azamfirei L,Mezei T,Cîmpean AM||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (48:349)||2007|
Significance of neo-angiogenesis and immuno-surveillance cells in squamous cell carcinoma of the tongue.
MA5-11854 was used in immunohistochemistry to study the prognostic value of microvessel, mast cell and eosinophil densities in squamous cell carcinoma of the tongue
|Alkhabuli JO||The Libyan journal of medicine (2:30)||2007|
Expression of NOS and HIF-1alpha in human colorectal carcinoma and implication in tumor angiogenesis.
MA5-11854 was used in immunohistochemistry to examine the role of nitric oxide synthase and hypoxia-inducible factor 1 in the pathogenesis of human colorectal carcinoma
|Yu JX,Cui L,Zhang QY,Chen H,Ji P,Wei HJ,Ma HY||World journal of gastroenterology (12:4660)||2006|
Correlation between CD105 expression and postoperative recurrence and metastasis of hepatocellular carcinoma.
MA5-11854 was used in immunohistochemistry to study the prognostic value of CD105 expression in postoperative recurrence and metastasis of hepatocellular carcinoma
|Yang LY,Lu WQ,Huang GW,Wang W||BMC cancer (6:null)||2006|
PDGFR-ß (+) perivascular cells from infantile hemangioma display the features of mesenchymal stem cells and show stronger adipogenic potential in vitro and in vivo.
MA5-11854 was used in immunocytochemistry to isolate and characterize PDGFR-beta(+) perivascular cells from infantile hemangioma
|Yuan SM,Guo Y,Zhou XJ,Shen WM,Chen HN||International journal of clinical and experimental pathology (7:2861)||2014|
Mesenchymal stem cells in infantile hemangioma reside in the perivascular region.
MA5-11854 was used in immunocytochemistry to test if MSCs in hemangioma also reside in the perivascular region
|Yuan SM,Chen RL,Shen WM,Chen HN,Zhou XJ||Pediatric and developmental pathology : the official journal of the Society for Pediatric Pathology and the Paediatric Pathology Society (15:5)||2012|
Isolation and culture of different epidermal and dermal cell types from human scalp suitable for the development of a therapeutical cell spray.
MA5-11854 was used in immunocytochemistry to investigate the usefulness of human scalp for epidermal and dermal cell preparation
|Schlabe J,Johnen C,Schwartlander R,Moser V,Hartmann B,Gerlach JC,Küntscher MV||Burns : journal of the International Society for Burn Injuries (34:376)||2008|
Isolation, characterization, and differentiation to hepatocyte-like cells of nonparenchymal epithelial cells from adult human liver.
MA5-11854 was used in immunocytochemistry to characterize nonparenchymal epithelial cells from adult human liver
|Duret C,Gerbal-Chaloin S,Ramos J,Fabre JM,Jacquet E,Navarro F,Blanc P,Sa-Cunha A,Maurel P,Daujat-Chavanieu M||Stem cells (Dayton, Ohio) (25:1779)||2007|
Cell fusion-independent differentiation of neural stem cells to the endothelial lineage.
MA5-11854 was used in immunocytochemistry to investigate the stem-cell plasticity of mouse neural stem cells
|Wurmser AE,Nakashima K,Summers RG,Toni N,D'Amour KA,Lie DC,Gage FH||Nature (430:350)||2004|
Endoglin requirement for BMP9 signaling in endothelial cells reveals new mechanism of action for selective anti-endoglin antibodies.
MA5-11854 was used in blocking or activating experiment to study the role of endoglin in endothelial cell BMP9 signaling and the anti-angiogenic effect of an anti-endoglin antibody
|Nolan-Stevaux O,Zhong W,Culp S,Shaffer K,Hoover J,Wickramasinghe D,Ruefli-Brasse A||PloS one (7:null)||2013|
HGF-transgenic MSCs can improve the effects of tissue self-repair in a rabbit model of traumatic osteonecrosis of the femoral head.
MA5-11854 was used in immunohistochemistry (paraffin) to discuss osteonecrosis of the femoral head.
|Wen Q,Jin D,Zhou CY,Zhou MQ,Luo W,Ma L||PloS one (7:null)||2012|
Expression of vascular endothelial growth factor in early cutaneous melanocytic lesion progression.
MA5-11854 was used in western blot to identify the therapeutic and prognostic markers for early cutaneous melanocytic lesion progression
|Einspahr JG,Thomas TL,Saboda K,Nickolof BJ,Warneke J,Curiel-Lewandrowski C,Ranger-Moore J,Duckett L,Bangert J,Fruehauf JP,Alberts DS||Cancer (110:2519)||2007|