Description: The MJ7/18 monoclonal antibody reacts with the mouse CD105 molecule, also known as Endoglin. This 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.
Applications Reported: The MJ7/18 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The MJ7/18 antibody has been tested by flow cytometric analysis of bEnd.3 cells and mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD105; Cell surface MJ7/18 antigen; Endoglin; ORW; transmembrane glycoprotein
Gene Aliases: AI528660; AI662476; CD105; Edg; Endo; Eng; S-endoglin
UniProt ID: (Mouse) Q63961
Entrez Gene ID: (Mouse) 13805