Invitrogen

CD105 (Endoglin) Monoclonal Antibody (SN6), eBioscience™

Product Image Gallery

CD105 (Endoglin) Antibody in Flow Cytometry (Flow) — Staining of U937 cell line with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or Anti-Human CD105 (Endoglin) Purified (filled histogram) followed by F (ab')2 Anti-Mouse IgG PE (Product # 12-4012). Total viable cells were used for analysis.

CD105 (Endoglin) Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Immunocytochemistry and Immunohistochemistry

CD105 (Endoglin) Antibody in Western Blot (WB) — Figure 7 ENG affects VEGFR2 trafficking and downstream signalling. ( a ) Immunoprecipitation of VEGFR2 with protein samples from VEGF-treated HDMECs lenti-virally transduced with either control shRNA or shRNA targeting ENG. Phosphorylated VEGFR2 was detected by blotting with pTyr antibody. ( b ) Ratio of Phosphorylated/total VEGFR2 was measured by ImageJ, n=4 independent experiments. ( c, d ) Western blot of total cell lysates of HDMECs, indicates knockdown of ENG in the sh ENG condition (non-reduced condition). VEGFA treatment for indicated time points reveals differential loss of VEGFR2 over time in control versus knockdown cells as shown by densitometric analysis relative to the loading control calnexin. P <0.001, 2-way ANOVA, n=3 independent experiments. ( e ) Total cell lysates of the above analysed for VEGFR2 downstream components p*AKT, pan AKT, p*ERK1/2 and the loading control calnexin. ( f, g ) Densitometric quantification of p*AKT/total AKT or p*ERK1/2 /total ERK showing potentiated AKT phosphorylation in ENG knockdown ECs ( P <0.001, 2-way ANOVA, n=7 independent experiments for time points 0, 10, 30 min; n=3 independent experiments for time points 5, 15, 20 min) and no difference in p* ERK1/2. ( h ) Cellular membrane proteins were isolated at different time points and analysed for VEGFR2 (note the absence of the intracellular unglycosylated band), ENG, p*Y and total amount (biotin). ( i ) Densitometric analysis of membrane localised VEGFR2 in ( h ). Red or green do

Product Details

14-1057-82

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
Flow Cytometry (Flow)	1 µg/test	View 31 publications 31 publications

Product Specifications
Species Reactivity	Human
Published species	Cat, Fruit fly, Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	SN6
Conjugate	Unconjugated APC APC-eFluor 780 eFluor 450 Functional Grade PE PE-Cyanine7 Super Bright 436 Super Bright 600 Super Bright 645
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2
Contains	0.09% sodium azide
Storage conditions	4° C
RRID	AB_467417

Product Specific Information

Description: The SN6 monoclonal antibody reacts with human CD105, also known as Endoglin. CD105, an approximately 90 kDa disulfide-linked homodimer is expressed by vascular endothelial cells and some bone marrow cells and activated macrophages. It is suggested that CD105 functions in adhesion and embryonic angiogenesis.

Applications Reported: The SN6 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The SN6 antibody has been tested by flow cytometric analysis of U937 cell line. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Therapeutic Effects of Human Urine-Derived Stem Cells in a Rat Model of Cisplatin-Induced Acute Kidney Injury In Vivo and In Vitro.

Stem cells international

Sun B,Luo X,Yang C,Liu P,Yang Y,Dong X,Yang Z,Xu J,Zhang Y,Li L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Development, characterization, and hematopoietic differentiation of Griscelli syndrome type 2 induced pluripotent stem cells.

Stem cell research & therapy

Güney-Esken G,Erol ÖD,Pervin B,Gürhan Sevinç G,Önder T,Bilgiç E,Korkusuz P,Günel-Özcan A,Uçkan-Çetinkaya D,Aerts-Kaya F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

The Effect of Angiotensin II, Retinoic Acid, EGCG, and Vitamin C on the Cardiomyogenic Differentiation Induction of Human Amniotic Fluid-Derived Mesenchymal Stem Cells.

International journal of molecular sciences

Gasiūnienė M,Valatkaitė E,Navakauskaitė A,Navakauskienė R

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu Nov 19 00:00:00 EST 2020

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Stem cells international

Zhao AG,Shah K,Freitag J,Cromer B,Sumer H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2020

ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells.

Cell discovery

Wang S,Hu B,Ding Z,Dang Y,Wu J,Li D,Liu X,Xiao B,Zhang W,Ren R,Lei J,Hu H,Chen C,Chan P,Li D,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CRISPR/Cas9-based genetic correction for recessive dystrophic epidermolysis bullosa.

NPJ Regenerative medicine

Webber BR,Osborn MJ,McElroy AN,Twaroski K,Lonetree CL,DeFeo AP,Xia L,Eide C,Lees CJ,McElmurry RT,Riddle MJ,Kim CJ,Patel DD,Blazar BR,Tolar J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

PF-127 hydrogel plus sodium ascorbyl phosphate improves Wharton's jelly mesenchymal stem cell-mediated skin wound healing in mice.

Stem cell research & therapy

Deng Q,Huang S,Wen J,Jiao Y,Su X,Shi G,Huang J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Fri Apr 03 00:00:00 EDT 2020

Chondrogenic Differentiation from Induced Pluripotent Stem Cells Using Non-Viral Minicircle Vectors.

Cells

Rim YA,Nam Y,Park N,Jung H,Lee K,Lee J,Ju JH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Telomere-dependent and telomere-independent roles of RAP1 in regulating human stem cell homeostasis.

Protein & cell

Zhang X,Liu Z,Liu X,Wang S,Zhang Y,He X,Sun S,Ma S,Shyh-Chang N,Liu F,Wang Q,Wang X,Liu L,Zhang W,Song M,Liu GH,Qu J

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis.

Nature communications

Deng L,Ren R,Liu Z,Song M,Li J,Wu Z,Ren X,Fu L,Li W,Zhang W,Guillen P,Izpisua Belmonte JC,Chan P,Qu J,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Fri Jul 26 00:00:00 EDT 2019

Human umbilical cord-derived mesenchymal stem cells ameliorate the enteropathy of food allergies in mice.

Experimental and therapeutic medicine

Yan N,Xu J,Zhao C,Wu Y,Gao F,Li C,Zhou W,Xiao T,Zhou X,Shao Q,Xia S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

14-1057 was used in Immunohistochemistry-immunofluorescence to determine whether murine adipose tissue can be used as an accurate comparative model of human disease, by comparing its architecture again human abdominal and gluteofemoral adipose tissue.

Mon Oct 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Mon Oct 01 00:00:00 EDT 2018

Utilizing Confocal Microscopy to Characterize Human and Mouse Adipose Tissue.

Tissue engineering. Part C, Methods

Blackshear CP,Borrelli MR,Shen EZ,Ransom RC,Chung NN,Vistnes SM,Irizarry D,Nazerali R,Momeni A,Longaker MT,Wan DC

Mon Oct 01 00:00:00 EDT 2018

The ratio of ADSCs to HSC-progenitors in adipose tissue derived SVF may provide the key to predict the outcome of stem-cell therapy.

Clinical and translational medicine

Kilinc MO,Santidrian A,Minev I,Toth R,Draganov D,Nguyen D,Lander E,Berman M,Minev B,Szalay AA

14-1057 was used in Flow cytometry/Cell sorting to open the way to future rational design of new treatment regimens for individuals by adjusting the cell ratio before the treatment.

Wed Feb 07 00:00:00 EST 2018

Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling.

Nature cell biology

Jin Y,Muhl L,Burmakin M,Wang Y,Duchez AC,Betsholtz C,Arthur HM,Jakobsson L

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Western Blot

Thu Jun 01 00:00:00 EDT 2017

Human umbilical cord mesenchymal stem cells improve the reserve function of perimenopausal ovary via a paracrine mechanism.

Stem cell research & therapy

Li J,Mao Q,He J,She H,Zhang Z,Yin C

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu Mar 09 00:00:00 EST 2017

Mesenchymal Stem Cell-Derived Extracellular Vesicles as Mediators of Anti-Inflammatory Effects: Endorsement of Macrophage Polarization.

Stem cells translational medicine

Lo Sicco C,Reverberi D,Balbi C,Ulivi V,Principi E,Pascucci L,Becherini P,Bosco MC,Varesio L,Franzin C,Pozzobon M,Cancedda R,Tasso R

14-1057 was used in Flow Cytometry to indicate that MSC-EVs possess effective anti-inflammatory properties, making them potential therapeutic agents more handy and safe than MSCs.

Wed Mar 01 00:00:00 EST 2017

Lymphatic Contribution to the Cellular Niche in Heterotopic Ossification.

Annals of surgery

Loder S,Agarwal S,Sorkin M,Breuler C,Li J,Peterson J,Gardenier J,Hsieh HH,Wang SC,Mehrara BJ,Levi B

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

MIF Plays a Key Role in Regulating Tissue-Specific Chondro-Osteogenic Differentiation Fate of Human Cartilage Endplate Stem Cells under Hypoxia.

Stem cell reports

Yao Y,Deng Q,Song W,Zhang H,Li Y,Yang Y,Fan X,Liu M,Shang J,Sun C,Tang Y,Jin X,Liu H,Huang B,Zhou Y

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Angiogenic activity mediates bone repair from human pluripotent stem cell-derived osteogenic cells.

Scientific reports

Zou L,Chen Q,Quanbeck Z,Bechtold JE,Kaufman DS

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Wed Mar 16 00:00:00 EDT 2016

Influence of aging on the quantity and quality of human cardiac stem cells.

Scientific reports

Nakamura T,Hosoyama T,Kawamura D,Takeuchi Y,Tanaka Y,Samura M,Ueno K,Nishimoto A,Kurazumi H,Suzuki R,Ito H,Sakata K,Mikamo A,Li TS,Hamano K

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Mon Mar 07 00:00:00 EST 2016

SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2.

Cell research

Pan H,Guan D,Liu X,Li J,Wang L,Wu J,Zhou J,Zhang W,Ren R,Zhang W,Li Y,Yang J,Hao Y,Yuan T,Yuan G,Wang H,Ju Z,Mao Z,Li J,Qu J,Tang F,Liu GH

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Tue Jan 19 00:00:00 EST 2016

Impaired function of bone marrow stromal cells in systemic mastocytosis.

Stem cell research

Nemeth K,Wilson TM,Ren JJ,Sabatino M,Stroncek DM,Krepuska M,Bai Y,Robey PG,Metcalfe DD,Mezey E

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells.

International journal of molecular sciences

Umezaki Y,Hashimoto Y,Nishishita N,Kawamata S,Baba S

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

Human adipose-derived stem cells partially rescue the stroke syndromes by promoting spatial learning and memory in mouse middle cerebral artery occlusion model.

Stem cell research & therapy

Zhou F,Gao S,Wang L,Sun C,Chen L,Yuan P,Zhao H,Yi Y,Qin Y,Dong Z,Cao L,Ren H,Zhu L,Li Q,Lu B,Liang A,Xu GT,Zhu H,Gao Z,Ma J,Xu J,Chen X

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Sat May 09 00:00:00 EDT 2015

Modeling familial cancer with induced pluripotent stem cells.

Cell

Lee DF,Su J,Kim HS,Chang B,Papatsenko D,Zhao R,Yuan Y,Gingold J,Xia W,Darr H,Mirzayans R,Hung MC,Schaniel C,Lemischka IR

Published figure using CD105 (Endoglin) monoclonal antibody (Product # 14-1057-82) in Flow Cytometry

Thu Apr 09 00:00:00 EDT 2015

Feline foamy virus adversely affects feline mesenchymal stem cell culture and expansion: implications for animal model development.

Stem cells and development

Arzi B,Kol A,Murphy B,Walker NJ,Wood JA,Clark K,Verstraete FJ,Borjesson DL

14-1057 was used in Flow cytometry/Cell sorting to investigate the use of mesenchymal stem cell therapy for a chronic oral inflammatory disease in cats.

Wed Apr 01 00:00:00 EDT 2015

Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.

Cell death & disease

Feng Y,Zhu M,Dangelmajer S,Lee YM,Wijesekera O,Castellanos CX,Denduluri A,Chaichana KL,Li Q,Zhang H,Levchenko A,Guerrero-Cazares H,Quiñones-Hinojosa A